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Peptide Scanning-assisted Identification of a Monoclonal Antibody-recognized Linear B-cell Epitope
Published on: March 24, 2017
Epitope Mapping of Anti-Telomerase Reverse Transcriptase Monoclonal Antibodies
Yoshikazu Furusawa1,2, Shunsuke Itai1, Shinji Yamada1
11 Department of Antibody Drug Development, Tohoku University Graduate School of Medicine , Sendai, Japan .
Abstract:
Telomerase reverse transcriptase (TERT) is the main catalytic subunit of telomerase-associated protein machinery. Upregulation of TERT at the transcriptional level results in immortal cell phenotype associated with cancer. To date we have developed eight anti-TERT monoclonal antibodies (mAbs) (TMab-4, TMab-5, TMab-6, TMab-7, TMab-9, TMab-10, TMab-11, and TMab-12) by immunizing mice with synthetic peptides (302-QHHAGPPSTSRPPRPWDTPC-321) of TERT. We further characterized those epitopes using enzyme-linked immunosorbent assay, and here we discuss the critical epitope of an anti-TERT mAb, which is applicable for immunohistochemical analysis.
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