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Updated: Feb 7, 2026

Evaluation of Oxidative Stress in Biological Samples Using the Thiobarbituric Acid Reactive Substances Assay
Published on: May 12, 2020
Exploiting the Thiobarbituric Acid Scaffold for Antibacterial Activity
Anamika Sharma1, Sikabwe Noki1, Sizwe J Zamisa2
1School of Health Sciences, University of KwaZulu-Natal, University Road, Westville, Durban, 4000, South Africa.
Abstract:
Thiobarbituric acid (TBA) has been considered a privileged structure for developing antimicrobial agents. Diversity was obtained at positions N and at C5 through acylation, Schiff base formation, Knoevenagel condensation, and thioamide and enamine formation. The present work describes the synthesis of small libraries based on the TBA moiety and above-mentioned reactions. Preliminary antimicrobial activity screening of the prepared compounds against selected bacteria (both Gram-positive and -negative) showed the best results for the Boc-Phe-TBA derivative. These results could be useful for designing and building libraries based on other amino acids with distinct protecting groups.
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