Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

CRISPR and crRNAs02:53

CRISPR and crRNAs

19.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.1K
CRISPR01:59

CRISPR

58.0K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
58.0K
What is Genetic Engineering?00:49

What is Genetic Engineering?

80.3K
Overview
80.3K
What are Viruses?00:50

What are Viruses?

128.3K
Overview
128.3K
Resistivity01:22

Resistivity

4.6K
When a voltage is applied to a conductor, an electrical field is generated, and charges in the conductor feel the force due to the electrical field. The current density that results depends on the electrical field and the properties of the material. In some materials, including metals at a given temperature, the current density is approximately proportional to the electrical field. In these cases, the current density can be modeled as:
4.6K
Resistance01:19

Resistance

6.0K
When a current moves through any conductor, the conductor causes some level of difficulty for the current to flow. The measure of that difficulty is known as the resistance of the material and is represented by R. Every material has its own resistance. In the case of conductors, heat is emitted whenever a current passes through them. Resistance depends on the resistivity of the material. Resistivity is a characteristic of the material used to fabricate electrical components, whereas the...
6.0K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Efficient site-specific gene addition using R2 retrotransposons in tobacco and rice.

Nature biotechnology·2026
Same author

Multivalent Display of Antimicrobial Peptides on Plant Virus Scaffolds Enhances Killing of Drug-Resistant Bacteria.

ACS nano·2026
Same author

Programmable Nucleic Acid Sensing in Human Cells Using Circularizable ssDNA.

Nature communications·2026
Same author

Characterization and engineering of highly efficient Cas12j genome editors.

Trends in biotechnology·2026
Same author

Engineered base editors with reduced bystander editing through directed evolution.

Nature biotechnology·2025
Same author

Plasmid2MC: efficient cell-free generation of high-purity minicircle DNA for genome editing in mammalian cells.

Communications biology·2025

Related Experiment Video

Updated: Feb 7, 2026

Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

28.0K

Engineering virus resistance via CRISPR-Cas systems.

Ahmed Mahas1, Magdy Mahfouz1

  • 1Laboratory for Genome Engineering, Division of Biological Sciences, 4700 King Abdullah University of Science and Technology, Thuwal 23955-6900, Saudi Arabia.

Current Opinion in Virology
|July 14, 2018
PubMed
Summary
This summary is machine-generated.

CRISPR/Cas systems offer powerful antiviral defense in eukaryotes by targeting viral nucleic acids. These systems are also used in screens to uncover virus-host interactions and potential therapeutic targets.

More Related Videos

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems
10:52

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Published on: October 14, 2025

680
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.3K

Related Experiment Videos

Last Updated: Feb 7, 2026

Substrate Generation for Endonucleases of CRISPR/Cas Systems
11:53

Substrate Generation for Endonucleases of CRISPR/Cas Systems

Published on: September 8, 2012

28.0K
Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems
10:52

Precise Phage Mutagenesis with NgTET-Assisted CRISPR-Cas Systems

Published on: October 14, 2025

680
Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins
10:46

Gene Digital Circuits Based on CRISPR-Cas Systems and Anti-CRISPR Proteins

Published on: October 18, 2022

2.3K

Area of Science:

  • Molecular Biology
  • Virology
  • Immunology

Background:

  • CRISPR/Cas systems provide adaptive immunity in prokaryotes against foreign genetic elements.
  • The native function of CRISPR/Cas has been repurposed for antiviral strategies in eukaryotic organisms.
  • CRISPR/Cas9 and CRISPR/Cas13 systems are engineered to target DNA and RNA viruses, respectively.

Purpose of the Study:

  • To review recent advancements in utilizing CRISPR/Cas systems for combating eukaryotic viral infections.
  • To identify and discuss significant challenges and future directions in this field.
  • To explore the application of CRISPR/Cas in genome-wide screens for understanding virus-host interactions.

Main Methods:

  • Discussion of recent literature on CRISPR/Cas-based antiviral approaches in eukaryotes.
  • Analysis of engineered CRISPR/Cas9 and CRISPR/Cas13 systems for DNA and RNA virus targeting.
  • Review of CRISPR/Cas applications in genome-wide screens to identify host factors.

Main Results:

  • Engineered CRISPR/Cas systems demonstrate efficacy in conferring resistance against DNA and RNA viruses in eukaryotes.
  • CRISPR/Cas-based screens are instrumental in identifying critical host factors involved in viral infections.
  • These systems enhance the understanding of fundamental virus biology and virus-host interactions.

Conclusions:

  • CRISPR/Cas technology presents a promising avenue for developing novel antiviral therapies in eukaryotes.
  • Further research is needed to overcome challenges and fully realize the potential of CRISPR/Cas in combating viral diseases.
  • CRISPR/Cas applications in screens are crucial for dissecting complex virus-host dynamics.