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Modulation of E-receptor expression on activated T lymphocytes.

S K Oh, W L Farrar, F W Ruscetti

    Clinical Immunology and Immunopathology
    |January 1, 1986
    PubMed
    Summary

    Newly synthesized E-receptors on T lymphocytes form stable E-rosettes. Their expression, induced by T-cell activators, parallels Tac antigen, potentially marking activated T lymphocytes.

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    Area of Science:

    • Immunology
    • Cell Biology

    Background:

    • The E-receptor's role in T lymphocyte activation and function is not fully understood.
    • Identifying markers for activated T lymphocytes is crucial for immunological research.

    Purpose of the Study:

    • To investigate the induction and characteristics of new E-receptor expression on T lymphocytes.
    • To determine if E-receptor expression can serve as a marker for activated T lymphocytes.

    Main Methods:

    • Quantification of E-receptor expression using 125I-labeled monoclonal anti-E receptor antibody.
    • Culture of T lymphocytes with various activators (phytohemagglutinin, phorbol myristate acetate, lipopolysaccharide) and cytokines (Interleukins 1 and 2, Interferon-gamma).
    • Assessment of Tac antigen expression and endorphin's effect on E-receptor modulation.

    Main Results:

    • Newly synthesized E-receptors on activated T lymphocytes mediate stable E-rosette formation at 37°C.
    • Maximum E-receptor expression required 50 hours of culture with polyclonal T-cell activators; lipopolysaccharide did not induce expression.
    • E-receptor expression paralleled Tac antigen induction, but not by Interleukins or Interferon-gamma.
    • Endorphin showed a biphasic effect on E-receptor expression in the absence of polyclonal activators.

    Conclusions:

    • Induction of new E-receptor expression is a specific response to T-cell activation.
    • E-receptor expression, similar to Tac antigen, may serve as a reliable marker for activated T lymphocytes.

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