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Related Experiment Video

Updated: Feb 7, 2026

Laser Microdissection-Based Protocol for the LC-MS/MS Analysis of the Proteomic Profile of Neuromelanin Granules

Published on: December 16, 2021

Proteomics Analysis of Myelin Composition.

Akihiro Ishii¹, David Han¹, Rashmi Bansal²

¹Department of Neuroscience, University of Connecticut Medical School, Farmington, CT, USA.

Methods in Molecular Biology (Clifton, N.J.)

|July 15, 2018

Summary

This summary is machine-generated.

Researchers purified myelin proteins from brains to identify key components. This analysis advances understanding of myelination and myelin disease mechanisms.

Keywords:

Liquid chromatography Myelin Proteomics Sucrose gradient Tandem mass spectrometry

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Quantitative Analysis of Chromatin Proteomes in Disease

Quantitative Analysis of Chromatin Proteomes in Disease

Published on: December 28, 2012

Area of Science:

Neuroscience
Proteomics
Biochemistry

Background:

Myelin proteins are crucial for understanding myelination and myelin-related diseases.
A comprehensive identification of the myelin proteome is needed.

Purpose of the Study:

To establish a procedure for myelin purification from rodent and human brains.
To perform large-scale proteomic analysis of myelin.

Main Methods:

Myelin purification from brain tissue.
Proteomic analysis using one-dimensional polyacrylamide gel electrophoresis (PAGE).
Liquid chromatography (LC)/tandem mass spectrometry (MS) for protein identification.

Main Results:

A detailed procedure for myelin purification was developed.
A large-scale analysis of the myelin proteome was successfully performed.
The study identified a repertoire of myelin proteins.

Conclusions:

The developed procedure enables robust myelin purification and proteomic analysis.
This work provides a foundation for studying myelin function and disease pathogenesis.
The identified myelin proteome aids in understanding neurological disorders.