Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

GTPases and their Regulation02:14

GTPases and their Regulation

9.9K
Guanine nucleotide-binding proteins (G-proteins), also known as GTPases, are a superfamily of proteins that regulate many cellular processes, such as cell signaling, vesicular transport, and the regulation of cell shape and motility. Mutation or dysfunction of these proteins can lead to disease. There are around 40,000 known G-proteins that can broadly be classified into two groups ‒  small G-proteins consisting of a single domain and large multi-domain G-proteins.
Large G-proteins,...
9.9K
C4 Pathway and CAM01:27

C4 Pathway and CAM

49.2K
Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
49.2K
Epigenetic Regulation01:46

Epigenetic Regulation

33.9K
Epigenetic mechanisms play an essential role in healthy development. Conversely, precisely regulated epigenetic mechanisms are disrupted in diseases like cancer.
33.9K
Regulated Protein Degradation02:58

Regulated Protein Degradation

8.9K
It is vital to regulate the activity of enzymatic as well as non-enzymatic proteins inside the cell. This can be achieved either through creating a balance between their rate of synthesis and degradation or regulating the intrinsic activity of the protein. Both these regulation mechanisms play an essential role in the normal functioning of cells.
Protein degradation plays two important roles in the cells. It helps to protect cells from misfolded or damaged proteins before they lead to a...
8.9K
Master Transcription Regulators02:23

Master Transcription Regulators

7.8K
Master transcription regulators are regulatory proteins that are predominantly responsible for regulating the expression of multiple genes. Often these genes work in concert to drive a  complex process. Activation of a master transcription regulator can lead to a cascade of transcriptional activation necessary for that outcome. These regulators can directly bind to the regulatory sequences of the various genes involved, or they can indirectly regulate transcription by binding to regulatory...
7.8K
Negative Regulator Molecules01:23

Negative Regulator Molecules

38.6K
Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
38.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Ferroptosis is a Physiologic Vulnerability of Iron-Recycling Macrophages.

bioRxiv : the preprint server for biology·2026
Same author

Endothelial KLF4 depletion drives age-related neurovascular dysfunction and neuropsychiatric impairment.

Proceedings of the National Academy of Sciences of the United States of America·2026
Same author

Multifaceted roles for persulfide species in redox chemical biology.

Nature chemical biology·2026
Same author

Biliverdin Reductase Catalytic Activity Is Essential for Malaria Resistance.

bioRxiv : the preprint server for biology·2026
Same author

Cystathionine γ-lyase is a major regulator of cognitive function through neurotrophin signaling and neurogenesis.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Biliverdin reductase A is a major determinant of protective NRF2 signaling.

Proceedings of the National Academy of Sciences of the United States of America·2025

Related Experiment Video

Updated: Feb 7, 2026

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells
10:43

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

Published on: May 24, 2014

11.8K

Regulators of the transsulfuration pathway.

Juan I Sbodio1, Solomon H Snyder1,2,3, Bindu D Paul1

  • 1The Solomon H. Snyder Department of Neuroscience, The Johns Hopkins University School of Medicine, Baltimore, MD, USA.

British Journal of Pharmacology
|July 15, 2018
PubMed
Summary

The transsulfuration pathway regulates sulfur metabolism, producing cysteine and hydrogen sulfide (H₂S). Its dysregulation is linked to diseases, but targeting this pathway offers therapeutic potential.

More Related Videos

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity
07:09

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

8.0K
A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information
05:01

A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information

Published on: July 1, 2020

3.8K

Related Experiment Videos

Last Updated: Feb 7, 2026

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells
10:43

siRNA Screening to Identify Ubiquitin and Ubiquitin-like System Regulators of Biological Pathways in Cultured Mammalian Cells

Published on: May 24, 2014

11.8K
Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity
07:09

Myeloid Innate Signaling Pathway Regulation by MALT1 Paracaspase Activity

Published on: January 7, 2019

8.0K
A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information
05:01

A Pathway Association Study Tool for GWAS Analyses of Metabolic Pathway Information

Published on: July 1, 2020

3.8K

Area of Science:

  • Biochemistry
  • Metabolic pathways
  • Chemical Biology

Background:

  • The transsulfuration pathway is crucial for cellular function, generating key metabolites like cysteine and hydrogen sulfide (H₂S).
  • Precise regulation of cystathionine β-synthase and cystathionine γ-lyase is essential for pathway control.
  • Disruptions in this pathway are implicated in vascular dysfunction, Huntington's disease, and aging.

Purpose of the Study:

  • To review the regulation of the transsulfuration pathway, focusing on cysteine and H₂S.
  • To explore the therapeutic potential of targeting this pathway for novel treatments.

Main Methods:

  • Literature review of the transsulfuration pathway.
  • Analysis of regulatory mechanisms of key enzymes.
  • Examination of the role of cysteine and H₂S in disease pathology.

Main Results:

  • The transsulfuration pathway is tightly regulated at multiple enzymatic levels.
  • Cysteine and H₂S are critical metabolites with significant physiological roles.
  • Therapeutic strategies involving H₂S donors or pathway stimulation show promise.

Conclusions:

  • Understanding the regulation of the transsulfuration pathway is key to developing new treatments.
  • Targeting cysteine and H₂S metabolism offers a promising avenue for therapeutic intervention in various disorders.