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Conductometric biosensor for arginine determination in pharmaceutics
O V Soldatkina1, O O Soldatkin2, T P Velychko1
1Taras Shevchenko National University of Kyiv, Volodymyrska Str., 64, 01003 Kyiv, Ukraine.
Bioelectrochemistry (Amsterdam, Netherlands)
|July 15, 2018
Summary
A novel conductometric biosensor coimmobilizing urease and arginase enables precise arginine determination in pharmaceuticals. This optimized biosensor offers high sensitivity and stability for drug analysis.
Area of Science:
- Biotechnology
- Analytical Chemistry
- Biomedical Engineering
Background:
- Arginine is a crucial amino acid in pharmaceutical formulations.
- Accurate determination of arginine is essential for drug quality control.
- Existing analytical methods may lack the sensitivity or efficiency required for pharmaceutical analysis.
Purpose of the Study:
- To develop and optimize a conductometric biosensor for sensitive and selective arginine determination.
- To evaluate the analytical performance and stability of the developed biosensor.
- To apply the biosensor for quantifying arginine in pharmaceutical products.
Main Methods:
- Coimmobilization of urease and arginase enzymes onto a conductometric sensor.
- Optimization of immobilization parameters (enzyme and glutaraldehyde concentrations, incubation time).
- Optimization of solution parameters (pH, ionic strength, buffer capacity, Mn2+ concentration).
Main Results:
- Optimized biosensor exhibited a limit of detection of 2.5 μM and a linear range of 2.5-500 μM.
- Achieved high sensitivity (13.4 ± 2.4 μS/mM) and rapid response time (20 s).
- Demonstrated good repeatability, operational stability over a week, and selectivity against other amino acids.
Conclusions:
- The developed conductometric biosensor is effective for arginine determination in pharmaceutical samples.
- The biosensor provides accurate and reliable results, correlating well with manufacturer specifications.
- This technology offers a promising tool for quality control in the pharmaceutical industry.
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