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Related Concept Videos

Bone Disorders01:29

Bone Disorders

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Aging and its effect on bone remodeling is the most common cause of bone disorders. In young and healthy people, bone deposition and resorption happen at an equal rate to maintain optimal bone health.
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Bone Marrow Sampling and Transplants01:22

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Bone marrow transplant is a potential cure for several diseases, including cancer and specific genetic disorders. Notably, this procedure is applicable for patients suffering from aplastic anemia, certain types of leukemia, severe combined immunodeficiency disease (SCID), Hodgkin's disease, non-Hodgkin's lymphoma, multiple myeloma, thalassemia, sickle-cell disease, and certain cancers.
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Cell Specific Gene Expression01:58

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Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
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Intrinsically Disordered Proteins02:18

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Intrinsically disordered proteins are a group of proteins that do not fold into specific three-dimensional structures. Their structural flexibility allows them to complement ordered proteins to perform functions that are inaccessible to rigid structures. They are more common in eukaryotes than prokaryotes and may either be exclusively intrinsically disordered or hybrid proteins, consisting of a mix of ordered and disordered regions. The absence of a rigid structure in these proteins can be...
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Bone Cells and Tissue01:30

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Bones contain a relatively small number of cells entrenched in a matrix of organic and inorganic components. Although bone cells compose only a small amount of the bone volume, they are crucial to its function. Four types of cells are found within the bone tissue— osteoblasts, osteocytes, osteogenic cells, and osteoclasts.
Osteoblasts and Osteocytes
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KIT mutation and AHN-associated oncogenic profiles in systemic mastocytosis with an associated hematological neoplasm.

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Related Experiment Video

Updated: Feb 7, 2026

The Preparation of Primary Hematopoietic Cell Cultures From Murine Bone Marrow for Electroporation
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Bone Marrow Expression of Mast Cell Disorders.

Laura Sánchez-Muñoz1, Ana Filipa Henriques1, Iván Alvarez-Twose1

  • 1Instituto de Estudios de Mastocitosis de Castilla La Mancha, Hospital Virgen del Valle, Ctra. Cobisa s/n, Toledo 45071, Spain; Spanish Network on Mastocytosis (REMA), Toledo, Spain.

Immunology and Allergy Clinics of North America
|July 16, 2018
PubMed
Summary

Diagnosing rare mast cell disorders is challenging. Bone marrow analysis using morphologic, immunophenotypic, and molecular studies is crucial for accurate diagnosis and classification of systemic mastocytosis.

Keywords:
Bone marrowCell purificationDiagnosisFlow cytometryKIT mutationMast cellsMastocytosisPrognosis

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Area of Science:

  • Hematology
  • Rare Diseases
  • Oncology

Background:

  • Mast cell disorders are rare and heterogeneous, posing diagnostic challenges.
  • Accurate diagnosis is essential for effective patient management and treatment.
  • Systemic involvement often requires specialized diagnostic approaches.

Purpose of the Study:

  • To review key characteristics of mast cell disorders in bone marrow.
  • To outline methodological approaches for objective diagnosis and classification.
  • To improve the diagnostic workup for suspected systemic mastocytosis.

Main Methods:

  • Bone marrow analysis is the primary screening site.
  • Utilizes morphologic, immunohistochemical, and flow cytometric immunophenotyping.
  • Includes molecular studies for comprehensive evaluation.

Main Results:

  • Bone marrow examination provides critical diagnostic and prognostic information.
  • A multi-method approach ensures objective and reproducible classification.
  • Standardized diagnostic protocols are vital in reference centers.

Conclusions:

  • Bone marrow analysis is central to diagnosing mast cell disorders.
  • Integrated diagnostic methods enhance accuracy and reproducibility.
  • Expert centers are key for managing these rare conditions.