Related Experiment Video
Updated: Feb 7, 2026

Probe-based Real-time PCR Approaches for Quantitative Measurement of microRNAs
Published on: April 14, 2015
A targetable fluorescent probe for real-time monitoring of fluoride ions in mitochondria
Kai Zhou1, Mingguang Ren1, Li Wang1
1Institute of Fluorescent Probes for Biological Imaging, School of Chemistry and Chemical Engineering, School of Materials Science and Engineering, University of Jinan, Jinan, Shandong 250022, PR China.
Abstract:
Fluorion are pivotal anions in biology because they play an important role in dental care, treating osteoporosis, preventing tooth decay and promoting the healthy growth of bone. Studies have shown that high levels of fluoride will lead to the inactivation of the mitochondria. Therefore, it is urgent to develop a method to detect the fluoride anions in the mitochondria. Herein, we have developed a novel mitochondrial-target fluorescent probe for detecting F- in living cells. The probe exhibited excellent sensitivity and high selectivity for F- over the other relative species. With changing fluoride ions, the fluorescence spectrum of the probe changed significantly with a large turn-on fluorescence signal. Cell imaging indicated that the probe can penetrate viable cell membranes and rapidly detects and images fluorion over other anions in the mitochondria.
More Related Videos
08:32Application of Genetically Encoded Fluorescent Nitric Oxide (NO•) Probes, the geNOps, for Real-time Imaging of NO• Signals in Single Cells
Published on: March 16, 2017
12:23Real-time Monitoring of Ligand-receptor Interactions with Fluorescence Resonance Energy Transfer
Published on: August 20, 2012
Related Concept Videos
Ions and Ionic Charges
Peroxisomes and Mitochondria
The peroxisome is a single membrane-bound cellular organelle that can perform several different functions, including lipid metabolism and chemical detoxification. The enzymes within...
Trends in Lattice Energy: Ion Size and Charge
Real Time RT-PCR
The real-time quantification of the number of amplified products is...
Mitochondria
Common Ion Effect