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Updated: Feb 7, 2026

Multicolor Flow Cytometry-based Quantification of Mitochondria and Lysosomes in T Cells
Published on: January 9, 2019
A lysosome targetable fluorescent probe for palladium species detection base on an ESIPT phthalimide derivative
Youming Shen1, Xiangyang Zhang1, Yanyang Wu2
1Hunan Province Cooperative Innovation Center for The Construction & Development of Dongting Lake Ecological Economic Zone, Collaborative innovation center for efficient and health production of fisheries in Hunan province, College of Chemistry and Materials Engineering, Hunan University of Arts and Science, Changde 415000, PR China.
Abstract:
A novel lysosome-targetable phthalimide fluorescent probe was designed for detecting palladium based on ESIPT for signal transduction. The fluorescent probe conjugating with allylcarbamate displayed weak fluorescent due to the ESIPT process hinder by allylcarbamate. But with the addition of palladium, the ESIPT emission was recovery though the palladium-catalyzed deallylation reaction and the fluorescence intensity exhibited 40-fold enhancement at 511 nm. In addition, the probe showed excellent selectivity, high sensitivity, fast responds and low limit detection for palladium with a larger Stoke-shift. Moreover, the targetable probe was also successfully applied for detecting palladium in lysosomes of living cells. Hence, the probe though ESIPT modulation is a promising for monitoring palladium in practical samples.
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