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Characterization of a novel phage infecting the pathogenic multidrug-resistant Bacillus cereus and functional

Qin Peng1, Yihui Yuan2

  • 1Ministry of Education Key Laboratory for Ecology of Tropical Islands, College of Life Sciences, Hainan Normal University, Haikou, 571158, People's Republic of China.

Applied Microbiology and Biotechnology
|July 16, 2018
PubMed

Insights

A novel bacteriophage, vB_BceM-HSE3, and its endolysin PlyHSE3 were identified to combat multidrug-resistant Bacillus cereus. These agents show broad lytic activity and stability, offering potential for phage therapy and food safety applications.

Area of Science:

  • Microbiology
  • Bacteriology
  • Molecular Biology

Background:

  • Bacillus cereus is a prevalent foodborne pathogen causing human illness.
  • The rise of multidrug-resistant B. cereus necessitates novel antimicrobial strategies.
  • Bacteriophages and their endolysins offer specific lytic activity against bacteria.

Purpose of the Study:

  • To isolate and characterize a novel bacteriophage targeting multidrug-resistant B. cereus.
  • To investigate the lytic activity and properties of the phage's endolysin.
  • To evaluate the potential of the phage and endolysin for therapeutic and food safety applications.

Main Methods:

  • Isolation and characterization of bacteriophage vB_BceM-HSE3 from a multidrug-resistant B. cereus strain.
  • Genomic analysis of the isolated phage.
  • Functional analysis of the phage endolysin PlyHSE3, including lytic spectrum, temperature, and pH tolerance assays.

Main Results:

  • Phage vB_BceM-HSE3 specifically infects B. cereus group strains (B. cereus, B. anthracis, B. thuringiensis) and exhibits high temperature/pH tolerance.
  • Genomic analysis confirmed vB_BceM-HSE3 as a novel phage with low genome similarity to known phages.
  • Endolysin PlyHSE3 demonstrated a broader lytic spectrum than the phage, lysing B. cereus group strains and P. aeruginosa, with significant temperature and pH stability.

Conclusions:

  • Phage vB_BceM-HSE3 and its endolysin PlyHSE3 represent promising antimicrobial agents against pathogenic B. cereus group strains.
  • PlyHSE3's broad lytic activity and stability at various temperatures (4°C to >45°C) suggest its utility in food preservation and safety.
  • The study contributes to understanding phage diversity and provides valuable resources for developing phage-based therapies.
Keywords:
Bacillus cereusEndolysinMultidrug resistancePhagePhage therapy

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