Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Constant Pressure Calorimetry03:02

Constant Pressure Calorimetry

97.8K
Calorimetry is a technique used to measure the amount of heat involved in a chemical or physical process or to measure the heat transferred to or from a substance. The heat is exchanged with a calibrated and insulated device called the calorimeter. Calorimetry experiments are based on the assumption that there is no heat exchange between the insulated calorimeter and the external environment. The well-insulated calorimeters prevent the transfer of heat between the calorimeter and its external...
97.8K
Calorimetry01:19

Calorimetry

4.5K
When objects at different temperatures are placed in contact with each other but isolated from everything else, they attain thermal equilibrium. A container that prevents heat transfer in or out is called a calorimeter, and the use of a calorimeter to make measurements is called calorimetry. Generally, these measurements involve heat or specific heat capacity. The term "calorimetry problem" is used for any problem where the specified objects are thermally isolated from their...
4.5K
Histone Modification02:32

Histone Modification

16.2K
The histone proteins have a flexible N-terminal tail extending out from the nucleosome. These histone tails are often subjected to post-translational modifications such as acetylation, methylation, phosphorylation, and ubiquitination. Particular combinations of these modifications form “histone codes” that influence the chromatin folding and tissue-specific gene expression.
Acetylation
The enzyme histone acetyltransferase adds acetyl group to the histones. Another enzyme, histone...
16.2K
Oxidation Numbers03:14

Oxidation Numbers

42.9K
In redox reactions, the transfer of electrons occurs between reacting species. Electron transfer is described by a hypothetical number called the oxidation number (or oxidation state). It represents the effective charge of an atom or element, which is assigned using a set of rules.
42.9K
Constant Volume Calorimetry02:41

Constant Volume Calorimetry

30.8K
Calorimeters are useful to determine the heat released or absorbed by a chemical reaction. Coffee cup calorimeters are designed to operate at constant (atmospheric) pressure and are convenient to measure heat flow (or enthalpy change) accompanying processes that occur in solution at constant pressure. A different type of calorimeter that operates at constant volume, colloquially known as a bomb calorimeter, is used to measure the energy produced by reactions that yield large amounts of heat and...
30.8K
Spreading of Chromatin Modifications02:25

Spreading of Chromatin Modifications

9.5K
The histone proteins in the nucleosomes are post-translationally modified (PTM) to increase or decrease access to DNA. The commonly observed PTMs are methylation, acetylation, phosphorylation, and ubiquitination of lysine amino acids in the histone H3 tail region. These histone modifications have specific meaning for the cell. Hence, they are called "histone code". The protein complex involved in histone modification is termed as "reader-writer" complex.
Writers
The writer...
9.5K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

A role of methionines in the functioning of oxidatively modified fibrinogen.

Biochimica et biophysica acta. Proteins and proteomics·2024
Same author

Peroxidase-Like Activity of Magnetic Nanoparticles in the Presence of Blood Proteins.

Doklady. Biochemistry and biophysics·2023
Same author

Peroxide-Induced Damage to Plasminogen Molecules.

Doklady. Biochemistry and biophysics·2021
Same author

Effect of Hypochlorite- and Peroxide-Induced Oxidation of Fibrinogen on the Fibrin Structure.

Doklady. Biochemistry and biophysics·2021
Same author

The Nature of Resistance of the Coagulation Factor XIII Structure to Hypochlorite-Induced Oxidation.

Doklady. Biochemistry and biophysics·2020
Same author

The Structural-Functional Damage of Fibrinogen Oxidized by Hydrogen Peroxide.

Doklady. Biochemistry and biophysics·2020
Same journal

Achieving Maximum Efficiency of A549 Cell Transfection with a Plasmid Delivered by Polyethylenimine-Based Polyplexes.

Doklady. Biochemistry and biophysics·2026
Same journal

Polymorphism of hOGG1 Gene and Susceptibility to Malignant Neoplasms in People Affected by Long-Term Low-Dose-Rate Exposure.

Doklady. Biochemistry and biophysics·2026
Same journal

Association between the rs1052133 Polymorphism of the OGG1 Gene and the Risk of Malignant Neoplasm Development in People Chronically Exposed to Radiation.

Doklady. Biochemistry and biophysics·2026
Same journal

Modular Nanotransporters Containing Anti-PSMA Nanobodies Are Able to Penetrate into the Nuclei of Prostate Cancer Cells.

Doklady. Biochemistry and biophysics·2026
Same journal

A New Model for the Transmembrane Heterocomplex of Hemagglutinin HA and Ionic Channel M2 Proteins in Influenza A Virus.

Doklady. Biochemistry and biophysics·2026
Same journal

Effect of Low Doses of Accelerated Carbon Ions with Energy of 450 MeV/Nucleon on Adaptive Response and Genetic Instability in Two Generations of Mice.

Doklady. Biochemistry and biophysics·2026
See all related articles

Related Experiment Video

Updated: Feb 7, 2026

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
08:13

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Published on: March 4, 2017

40.4K

Study of Human Fibrinogen Oxidative Modification using Differential Scanning Calorimetry.

M G Gorobets1, L A Wasserman2, A V Bychkova2

  • 1Emanuel Institute of Biochemical Physics, Russian Academy of Sciences, Moscow, 119334, Russia. maria.g.gorobets@gmail.com.

Doklady. Biochemistry and Biophysics
|July 16, 2018
PubMed
Summary
This summary is machine-generated.

Oxidative stress alters fibrinogen structure. Differential scanning calorimetry revealed that all key fibrinogen regions (D, αC, and E) undergo thermal denaturation changes when exposed to oxidation.

More Related Videos

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
13:26

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Published on: September 13, 2014

62.8K
An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
07:42

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

Published on: June 12, 2013

19.5K

Related Experiment Videos

Last Updated: Feb 7, 2026

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen
08:13

Differential Scanning Calorimetry — A Method for Assessing the Thermal Stability and Conformation of Protein Antigen

Published on: March 4, 2017

40.4K
Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry
13:26

Determination of Protein-ligand Interactions Using Differential Scanning Fluorimetry

Published on: September 13, 2014

62.8K
An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization
07:42

An In vitro Model to Study Heterogeneity of Human Macrophage Differentiation and Polarization

Published on: June 12, 2013

19.5K

Area of Science:

  • Biochemistry
  • Materials Science
  • Protein Chemistry

Background:

  • Fibrinogen is a key protein in blood coagulation.
  • Oxidative stress can alter protein structure and function.
  • Understanding fibrinogen's response to oxidation is crucial for studying thrombosis and related diseases.

Purpose of the Study:

  • To investigate the thermal denaturation of fibrinogen under induced oxidation for the first time.
  • To characterize structural changes in fibrinogen upon oxidation using differential scanning calorimetry.

Main Methods:

  • Differential scanning calorimetry (DSC) was employed to study thermal denaturation.
  • Induced oxidation was applied to fibrinogen samples.
  • Analysis of denaturation temperature, denaturation enthalpy, and van't Hoff enthalpy.

Main Results:

  • All detected fibrinogen structural elements (D region, αC-domain, and E region) are susceptible to oxidation.
  • Oxidation induced measurable changes in the thermal denaturation profiles of fibrinogen.
  • Specific parameters like denaturation temperature and enthalpy were altered by oxidation.

Conclusions:

  • Oxidation significantly impacts the structural integrity and thermal stability of fibrinogen.
  • DSC is a valuable tool for detecting oxidation-induced structural modifications in fibrinogen.
  • These findings contribute to understanding fibrinogen's role in oxidative stress-related conditions.