Related Experiment Video

Updated: Feb 7, 2026

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids
10:43

Modeling Oral-Esophageal Squamous Cell Carcinoma in 3D Organoids

Published on: December 23, 2022

4.1K

MicroRNA-1294 inhibited oral squamous cell carcinoma growth by targeting c-Myc

Zuojun Wang1, Jinsheng Yan2, Tingqian Zou1

  • 1Department of Stomatology, Stomatological Hospital of Jingmen City, Jingmen, Hubei 448000, P.R. China.

Oncology Letters
|July 17, 2018
PubMed

Insights

MicroRNA-1294 (miR-1294) acts as a tumor suppressor in oral squamous cell carcinoma (OSCC). Lower miR-1294 levels correlate with increased c-Myc, promoting OSCC growth and migration.

Area of Science:

  • Oncology
  • Molecular Biology
  • Genetics

Background:

  • Oral squamous cell carcinoma (OSCC) is a prevalent oral cancer subtype with a poor 5-year survival rate of approximately 50%.
  • The oncogene c-Myc is implicated in various cancers, including OSCC, highlighting its potential as a therapeutic target.
  • MicroRNAs (miRNAs) are small non-coding RNAs that regulate gene expression and play critical roles in cancer development.

Purpose of the Study:

  • To investigate the role of miR-1294 in oral squamous cell carcinoma (OSCC).
  • To determine the relationship between miR-1294 and the oncogene c-Myc in OSCC.
  • To elucidate the potential tumor suppressive function of miR-1294 in OSCC progression.

Main Methods:

  • Quantitative real-time PCR was used to measure miR-1294 and c-Myc mRNA expression levels in OSCC tissues and cell lines.
  • OSCC cell lines were transfected with miR-1294 mimics or inhibitors to assess the effects on proliferation and migration.
  • Bioinformatic analysis and luciferase reporter assays were employed to confirm the direct targeting of c-Myc by miR-1294.

Main Results:

  • miR-1294 expression was significantly lower in OSCC tissues compared to normal tissues.
  • Overexpression of miR-1294 suppressed proliferation and migration of OSCC cells, while inhibition of miR-1294 promoted these processes.
  • A negative correlation was observed between miR-1294 and c-Myc mRNA expression in OSCC tissues, indicating c-Myc is a direct target of miR-1294.

Conclusions:

  • miR-1294 functions as a tumor suppressor in oral squamous cell carcinoma (OSCC).
  • The miR-1294/c-Myc axis plays a crucial role in regulating OSCC cell proliferation and migration.
  • miR-1294 represents a potential therapeutic biomarker and target for OSCC treatment.
Keywords:
OSCCc-Myccell growthmiR-1294

More Related Videos

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice07:02

In Vivo Inhibition of MicroRNA to Decrease Tumor Growth in Mice

Published on: August 23, 2019

7.8K
Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma12:03

Multi-photon Imaging of Tumor Cell Invasion in an Orthotopic Mouse Model of Oral Squamous Cell Carcinoma

Published on: July 25, 2011

20.0K

Related Concept Videos

MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
24.3K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns (non-coding regions of a gene) or intergenic regions (stretches of DNA present between genes). Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself, forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After the pre-miRNA...
4.0K
Feedback Inhibition00:46

Feedback Inhibition

Biochemical reactions are occurring constantly in cells, converting starting substances to different products, usually with the help of enzymes that speed the reactions. Without enzymes, it would take far too long for most reactions to occur to be useful to the cell!
57.2K
Oral Cavity01:11

Oral Cavity

The oral cavity, or the mouth, is a complex structure in humans that plays a vital role in our day-to-day lives. Its role is not only in chewing and swallowing food; it also plays a role in speech and facial expressions.
Teeth: The teeth are the hardest structures in our bodies. Humans have two sets of teeth throughout their lifetime: deciduous (baby) teeth and permanent teeth. Each tooth consists of several parts: the crown (visible part), the root (embedded in the jaw), enamel (hard outer...
3.2K
Enzyme Inhibition01:30

Enzyme Inhibition

Inhibitors are molecules that reduce enzyme activity by binding to the enzyme. In a normally functioning cell, enzymes are regulated by a variety of inhibitors. Drugs and other toxins can also inhibit enzymes. Some inhibitors bind to the enzyme’s active site, while others inhibit enzymatic activity by binding to other sites on the protein structure.
92.6K
Primary and Secondary Growth in Roots and Shoots03:02

Primary and Secondary Growth in Roots and Shoots

Vascular plants, which account for over 90% of the Earth’s vegetation, all undergo primary growth—which lengthens roots and shoots. Many land plants, notably woody plants, also undergo secondary growth—which thickens roots and shoots.
60.6K