Mechanisms and regulation of epithelial phosphate transport in ruminants: approaches in comparative physiology

Ruminants have a unique utilization of phosphate (P_i) based on the so-called endogenous P_i recycling to guarantee adequate P_i supply for ruminal microbial growth and for buffering short-chain fatty acids. Large amounts of P_i enter the gastrointestinal tract by salivary secretion. The high saliva P_i concentrations are generated by active secretion of P_i from blood into primary saliva via basolateral sodium (Na⁺)-dependent P_i transporter type II. The following subsequent intestinal absorption of P_i is mainly carried out in the jejunum by the apical located secondary active Na⁺-dependent P_i transporters NaPi IIb (SLC34A2) and PiT1 (SLC20A1). A reduction in dietary P_i intake stimulates the intestinal P_i absorption by increasing the expression of NaPi IIb despite unchanged plasma 1,25-dihydroxyvitamin D₃ concentrations, which modulate P_i homeostasis in monogastric species. Reabsorption of glomerular filtrated plasma P_i is mainly mediated by the P_i transporters NaPi IIa (SLC34A1) and NaPi IIc (SLC34A3) in proximal tubule apical cells. The expression of NaPi IIa and the corresponding renal Na⁺-dependent P_i capacity were modulated by high dietary phosphorus (P) intake in a parathyroid-dependent manner. In response to reduced dietary P_i intake, the expression of NaPi IIa was not adapted indicating that renal P_i reabsorption in ruminants runs at a high level allowing no further increase when P intake is diminished. In bones and in the mammary glands, Na⁺-dependent P_i transporters are able to contribute to maintaining P_i homeostasis. Overall, the regulation of P_i transporter activity and expression by hormonal modulators confirms substantial differences between ruminant and non-ruminant species.