Related Experiment Video
Updated: Feb 7, 2026
08:19
High-Throughput Quantitative RT-PCR in Single and Bulk C. elegans Samples Using Nanofluidic Technology
Published on: May 28, 2020
8.5K
A novel mammalian cell line development platform utilizing nanofluidics and optoelectro positioning technology
Kim Le1, Christopher Tan1, Shivani Gupta1
1Drug Substance Technologies, Process Development, Amgen Inc., Thousand Oaks, CA, 91320.
Biotechnology Progress
|July 17, 2018
Summary
Generating high-yield cell lines is faster and cheaper using new nanofluidic technology. The Beacon platform enables high-throughput cell line development, reducing resource needs compared to traditional methods.
Area of Science:
- Biotechnology
- Cell Line Development
- Bioprocessing
Background:
- Cell line generation is resource-intensive and time-consuming.
- Miniaturization and automation are key to improving efficiency.
- Current methods use standard culture vessels and liquid handling equipment.
Purpose of the Study:
- To evaluate the Beacon platform for cell line development.
- To compare nanofluidic technology with traditional workflows.
- To demonstrate efficient isolation, culture, screening, and export of cell lines.
Main Methods:
- Utilizing Berkeley Lights' OptoElectro Positioning technology on nanofluidic chips.
- Programming cell culture tasks via software for parallel processing.
- Comparing the Beacon platform with FACS-enabled microtiter plate workflows.
Main Results:
- Commercial Chinese hamster ovary cell lines were successfully developed on the Beacon platform.
- High efficiency was achieved in isolating, culturing, screening, and exporting cell lines.
- Comparable clonal cell lines were generated with significantly reduced resources.
Conclusions:
- The Beacon platform offers a highly efficient and resource-saving approach to cell line development.
- Nanofluidic technology surpasses the limitations of conventional cell culture methods.
- This integrated platform streamlines the entire cell line development process.
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