Related Experiment Video

Updated: Feb 7, 2026

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay
09:07

Detecting the Ligand-binding Domain Dimerization Activity of Estrogen Receptor Alpha Using the Mammalian Two-Hybrid Assay

Published on: December 19, 2018

6.8K

Resveratrol represses estrogen-induced mammary carcinogenesis through NRF2-UGT1A8-estrogen metabolic axis activation

Xueyan Zhou1, Yun Zhao1, Juan Wang1

  • 1Jiangsu Key Laboratory of New Drug Research and Clinical Pharmacy, Xuzhou Medical University, Xuzhou, China.

Biochemical Pharmacology
|July 17, 2018
PubMed

Insights

Resveratrol prevents breast cancer by restoring estrogen balance. It upregulates NRF2-UGT1A8 signaling, enhancing catechol estrogen metabolism and reducing DNA damage.

Area of Science:

  • Oncology
  • Molecular Biology
  • Pharmacology

Background:

  • Estrogen is crucial in breast cancer development.
  • Resveratrol exhibits chemo-preventive properties, but its mechanism is unclear.
  • Estrogen homeostasis is disrupted in breast cancer, leading to carcinogenic catechol estrogen accumulation.

Purpose of the Study:

  • To elucidate the mechanism behind resveratrol's antitumor activity in breast cancer.
  • To investigate the role of the NRF2-UGT1A8 signaling pathway in estrogen metabolism and breast cancer.
  • To establish resveratrol's potential in regulating estrogen homeostasis via this pathway.

Main Methods:

  • Studied estrogen homeostasis in breast cancer patients and rat models.
  • Investigated the expression of UDP-glucuronosyltransferase 1A8 (UGT1A8) and nuclear factor erythroid 2-related factor 2 (NRF2).
  • Utilized resveratrol treatment, luciferase reporter assays, and small-interfering RNA (siRNA) for NRF2 silencing.

Main Results:

  • Breast cancer rat models showed down-regulated mammary NRF2-UGT1A8 signaling.
  • Resveratrol treatment upregulated NRF2 and UGT1A8, accelerating catechol estrogen metabolism.
  • Resveratrol inhibited estrogen-induced DNA damage and suppressed breast cancer development.
  • Luciferase assays confirmed resveratrol activates UGT1A8 via NRF2 transcriptional activity.
  • NRF2 silencing abolished resveratrol's preventive effects, highlighting the NRF2-UGT1A8-estrogen metabolism axis.

Conclusions:

  • Resveratrol's antitumor effects in breast cancer are mediated by the NRF2-UGT1A8-estrogen metabolism axis.
  • Resveratrol regulates estrogen homeostasis through the NRF2-UGT1A8 signaling pathway.
  • This study reveals a novel link between estrogen glucuronidation metabolism and breast cancer pathology.
Keywords:
Breast cancerEstrogenNuclear factor erythroid 2-related factor 2 (NRF2)ResveratrolUDP-Glucuronosyltransferase (UGT)

More Related Videos

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory14:13

Detecting Estrogenic Ligands in Personal Care Products using a Yeast Estrogen Screen Optimized for the Undergraduate Teaching Laboratory

Published on: January 1, 2018

13.8K
Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells16:24

Profiling of Estrogen-regulated MicroRNAs in Breast Cancer Cells

Published on: February 21, 2014

20.7K

Related Concept Videos

Repressed Memory01:16

Repressed Memory

Repressed memories are a psychological phenomenon where memories of traumatic events are unconsciously blocked from a person's awareness. This process occurs as a defense mechanism, protecting the mind from the emotional impact of distressing or painful experiences. For example, a person who has experienced childhood trauma may grow up with no conscious recollection of the event. In such cases, the memories are thought to be buried deep within the subconscious, inaccessible to the conscious...
526
Hypothalamic-Pituitary Axis01:37

Hypothalamic-Pituitary Axis

The response to stress—be it physical or psychological, acute or chronic—involves activation of the Hypothalamic-Pituitary-Adrenal (HPA) axis. The HPA axis is part of the neuroendocrine system because it involves both neuronal and hormonal communication. Its function is to regulate homeostatic systems—metabolic, cardiovascular, and immune—providing the necessary means to respond to a stressor.
66.3K
What is Metabolism?00:52

What is Metabolism?

Overview
132.0K
The Mammary Glands01:12

The Mammary Glands

The female breast is a hemispheric projection of variable size positioned anterior to the pectoralis major and serratus anterior muscles. A fascia layer composed of dense, irregular connective tissue connects it to these muscles.
Each breast features a pigmented projection known as the nipple, through which milk emerges via closely spaced openings of ducts, referred to as lactiferous ducts. Surrounding the nipple is a circular pigmented area of skin named the areola, which appears rough due to...
2.3K
Repressible Operon: trp Operon01:21

Repressible Operon: trp Operon

The trp operon in Escherichia coli exemplifies a repressible operon. It regulates the synthesis of tryptophan through repressor-mediated transcriptional control and attenuation. This dual regulatory mechanism ensures tryptophan biosynthesis occurs only when needed, conserving cellular resources.Structure of the trp OperonThe trp operon consists of five structural genes (trpE, trpD, trpC, trpB, and trpA) that encode enzymes for tryptophan biosynthesis. These genes are transcribed as a single...
1.7K
Perpendicular-Axis Theorem01:16

Perpendicular-Axis Theorem

The perpendicular-axis theorem states that the moment of inertia of a planar object about an axis perpendicular to its plane is equal to the sum of the moments of inertia about two mutually perpendicular concurrent axes lying in the plane of the body.
Consider a circular disc of mass M and radius R lying along an x-y plane. The origin lies at the center of the disc, and the z-axis is perpendicular to the disc's plane. All three axes coincide at the disc's center. The moment of inertia of this...
4.6K