Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Positive Regulator Molecules

Positive Regulator Molecules

To consistently produce healthy cells, the cell cycle—the process that generates daughter cells—must be precisely regulated.

Positive Regulator Molecules

Positive Regulator Molecules

Mitotic cell division results in daughter cells that exactly resemble the parent cell. However, errors in the DNA replication or distribution of genetic material may lead to genetic mutations that may be passed down to every new cell formed from the resulting abnormal cell. Propagation of such mutant cells is restricted through checkpoint mechanisms present at different stages of the cell cycle. These checkpoints involve regulator molecules that either promote or demote cell cycle events.

Chromatin Position Affects Gene Expression

Chromatin Position Affects Gene Expression

Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...

Regulation of Expression Occurs at Multiple Steps

Regulation of Expression Occurs at Multiple Steps

Gene expression can be regulated at almost every step from gene to protein. Transcription is the step that is most commonly regulated. This involves the binding of proteins to short regulatory sequences on the DNA. This association can either promote or inhibit the transcription of a gene associated with the respective sequence.
Transcription results in the generation of precursor (pre-mRNA) that consists of both exons and introns, which needs further processing before being translated to a...

Regulation of Expression Occurs at Multiple Steps

Regulation of Expression Occurs at Multiple Steps

Position-effect Variegation

Position-effect Variegation

In 1928, a German botanist Emil Heitz observed the moss nuclei with a DNA binding dye. He observed that while some chromatin regions decondense and spread out in the interphase nucleus, others do not. He termed them euchromatin and heterochromatin, respectively. He proposed that the heterochromatin regions reflect a functionally inactive state of the genome. It was later confirmed that heterochromatin is transcriptionally repressed, and euchromatin is transcriptionally active chromatin.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

GABAergic TH2 Amacrine Cells Participate in Spontaneous Wave Activity in the Developing Retina.

bioRxiv : the preprint server for biology·2026

Same author

A New Insight into the Study of Neural Cell Adhesion Molecule (NCAM) Polysialylation Inhibition Incorporated the Molecular Docking Models into the NMR Spectroscopy of a Crucial Peptide-Ligand Interaction.

Biomolecules·2026

Same author

Long-Term Impairment of Retinal Ganglion Cell Function After Oxygen-Induced Retinopathy.

Cells·2025

Same author

Downregulation of KLF9 alleviates tubulointerstitial fibrosis by modulating FABP4-mediated lipid accumulation.

IUBMB life·2025

Same author

A new mouse model for PRPH2 pattern dystrophy exhibits functional compensation prior and subsequent to retinal degeneration.

Human molecular genetics·2024

Same author

Dopaminergic amacrine cells express HCN channels in the developing and adult mouse retina.

bioRxiv : the preprint server for biology·2024

Search research articles

Related Experiment Video

Updated: Feb 7, 2026

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Published on: January 24, 2016

STING positively regulates human ORMDL3 expression through TBK1-IRF3-STAT6 complex mediation.

Qian Cao¹, Chen-Xia Juan¹, Dao-Qi Zhang¹

¹Department of Pediatrics, The First Affiliated Hospital, Nanjing Medical University, Nanjing 210029, Jiangsu Province, China.

Experimental Cell Research

|July 17, 2018

Summary

Stimulator of interferon gene (STING) positively regulates Orosomucoid 1-like protein 3 (ORMDL3) expression in asthma patients. This pathway involves STING activating TBK1, IRF3, and STAT6, which then upregulate ORMDL3.

Keywords:

Orosomucoid 1-like protein 3 (ORMDL3)Recurrent wheeze Stimulator of interferon genes (STING)TBK1-STAT6-IRF3 complex

More Related Videos

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Published on: July 16, 2011

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Published on: May 19, 2016

Related Experiment Videos

Last Updated: Feb 7, 2026

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

A High Resolution Method to Monitor Phosphorylation-dependent Activation of IRF3

Published on: January 24, 2016

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Lentiviral-mediated Knockdown During Ex Vivo Erythropoiesis of Human Hematopoietic Stem Cells

Published on: July 16, 2011

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Methods to Study Mrp4-containing Macromolecular Complexes in the Regulation of Fibroblast Migration

Published on: May 19, 2016

Area of Science:

Immunology
Genetics
Respiratory Medicine

Background:

Orosomucoid 1-like protein 3 (ORMDL3) is implicated in virus-triggered recurrent wheeze and asthma.
Stimulator of interferon gene (STING) is crucial for cytosolic antiviral responses.
The molecular link between STING and ORMDL3 in asthma pathogenesis remains undefined.

Purpose of the Study:

To investigate the association between STING and ORMDL3 in recurrent wheeze.
To elucidate the molecular mechanisms underlying the STING-ORMDL3 relationship.

Main Methods:

Correlation analysis of ORMDL3 and STING expression in patients.
Investigating the impact of STING on ORMDL3 transcriptional activity.
Assessing the roles of IRF3, STAT6, and TBK1 in the STING-ORMDL3 pathway.

Main Results:

ORMDL3 expression linearly correlates with STING levels in recurrent wheeze patients.
STING activation enhances ORMDL3 transcription via IRF3 and STAT6.
STING promotes IRF3 and STAT6 phosphorylation and binding through TBK1, leading to ORMDL3 promoter activation.

Conclusions:

STING positively regulates ORMDL3 expression in the context of recurrent wheeze.
The STING-TBK1-IRF3-STAT6 complex is a key mediator of ORMDL3 upregulation.
This pathway offers potential therapeutic targets for virus-induced asthma exacerbations.