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Lycorine: a eukaryotic termination inhibitor?

R Vrijsen, D A Vanden Berghe, A J Vlietinck

The Journal of Biological Chemistry

|January 15, 1986

Summary

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The alkaloid lycorine inhibits poliovirus protein synthesis in a dose-dependent manner, primarily affecting P3-derived proteins. This suggests lycorine may interfere with viral translation termination.

Area of Science:

Virology
Molecular Biology
Biochemistry

Background:

Poliovirus is a significant human pathogen.
Viral protein synthesis is crucial for viral replication.
Lycorine is an alkaloid with potential biological activities.

Purpose of the Study:

To investigate the effect of lycorine on poliovirus protein synthesis.
To determine the specific viral proteins affected by lycorine.
To elucidate the mechanism of lycorine's action on viral translation.

Main Methods:

Poliovirus-infected HeLa cells were used.
Incorporation of [3H]leucine was measured to assess protein synthesis.
Polyacrylamide gel electrophoresis was employed to identify viral proteins.

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Main Results:

Lycorine inhibited [3H]leucine incorporation in a dose-dependent manner.
Lycorine did not completely abolish viral translation.
Residual protein synthesis was mainly P1 and P2 proteins, with minimal P3 protein synthesis.

Conclusions:

Lycorine selectively inhibits the synthesis of P3-derived poliovirus proteins.
Lycorine's effect suggests a potential role in inhibiting viral translation termination.