Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

siRNA - Small Interfering RNAs02:30

siRNA - Small Interfering RNAs

18.7K
Small interfering RNAs, or siRNAs, are short regulatory RNA molecules that can silence genes post-transcriptionally, as well as the transcriptional level in some cases. siRNAs are important for protecting cells against viral infections and silencing transposable genetic elements.
In the cytoplasm, siRNA is processed from a double-stranded RNA, which comes from either endogenous DNA transcription or exogenous sources like a virus. This double-stranded RNA is then cleaved by the...
18.7K
piRNA - Piwi-interacting RNAs02:57

piRNA - Piwi-interacting RNAs

7.7K
PIWI-interacting RNAs, or piRNAs, are the most abundant short non-coding RNAs. More than 20,000 genes have been found in humans that code for piRNAs while only 2000 genes have been found for miRNAs. piRNAs can act at the transcriptional and post-transcriptional levels and have a vital role in silencing transposable elements present in germ cells. They are also involved in epigenetic silencing and activation. Previously, they were thought to function only in germ cells but new evidence suggests...
7.7K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

10.0K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
10.0K
lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

3.7K
3.7K
Small interfering RNAs (siRNA)02:30

Small interfering RNAs (siRNA)

4.6K
4.6K
Ribosome Profiling02:24

Ribosome Profiling

4.2K
Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
Applications of ribosome profiling
Ribosome profiling has many applications, including in vivo monitoring of translation inside a particular organ or tissue type and quantifying new protein synthesis levels.
The technique...
4.2K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Biliary Peritonitis Secondary to Proximal Duodenal Perforation in a Cat.

Veterinary radiology & ultrasound : the official journal of the American College of Veterinary Radiology and the International Veterinary Radiology Association·2026
Same author

Design and optimization of fully (100%) cellulose-based polyurethane foams: Strategies, challenges, and solutions for future applications.

International journal of biological macromolecules·2026
Same author

Excited-State Symmetry Breaking Dynamics Driven by Mode-Resolved Jahn-Teller Distortion in Luminescent Tris(2,4,6-trichlorophenyl)methyl Radicals.

Journal of the American Chemical Society·2026
Same author

Multimodal Imaging of Systemic Metastatic Myocardial and Vascular Calcification Associated with Renal Secondary Hyperparathyroidism in a Castrated Male Cat with End-Stage Chronic Kidney Disease: A Case Report.

Animals : an open access journal from MDPI·2026
Same author

Comparison of Volumetric Modulated Arc Therapy and Fixed-Field Intensity-Modulated Radiotherapy for Canine Prostatic Carcinoma Using a 4.3 Gy × 10-Fraction Protocol: A Dosimetric Planning Study.

Veterinary and comparative oncology·2026
Same author

Multicentric Round Cell Neoplasia with Plasmacytic Differentiation in a Cat with Systemic Progression: Multimodal Imaging and Treatment Response.

Animals : an open access journal from MDPI·2026

Related Experiment Video

Updated: Feb 7, 2026

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

9.3K

Profiling of testis-specific long noncoding RNAs in mice.

Seong Hyeon Hong1, Jun Tae Kwon1, Jihye Kim1

  • 1School of Life Sciences, Gwangju Institute of Science and Technology, Gwangju, 61005, Korea.

BMC Genomics
|July 18, 2018
PubMed
Summary

This study identifies novel long noncoding RNAs (lncRNAs) specific to the mouse testis. These findings provide a foundation for understanding the role of lncRNAs in spermatogenesis and male fertility.

Keywords:
Long noncoding RNASpermatogenesisTestisTestis-specific lncRNAscis-acting

More Related Videos

Isolation of Small Noncoding RNAs from Human Serum
06:44

Isolation of Small Noncoding RNAs from Human Serum

Published on: June 19, 2014

18.6K
Polysome Profiling in Leishmania, Human Cells and Mouse Testis
14:32

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Published on: April 8, 2018

18.9K

Related Experiment Videos

Last Updated: Feb 7, 2026

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR
13:04

Overexpressing Long Noncoding RNAs Using Gene-activating CRISPR

Published on: March 1, 2019

9.3K
Isolation of Small Noncoding RNAs from Human Serum
06:44

Isolation of Small Noncoding RNAs from Human Serum

Published on: June 19, 2014

18.6K
Polysome Profiling in Leishmania, Human Cells and Mouse Testis
14:32

Polysome Profiling in Leishmania, Human Cells and Mouse Testis

Published on: April 8, 2018

18.9K

Area of Science:

  • Genomics
  • Molecular Biology
  • Reproductive Biology

Background:

  • Spermatogenesis requires testis-specific transcripts for producing spermatozoa.
  • Long noncoding RNAs (lncRNAs) are emerging as critical regulatory molecules in biological processes.
  • A comprehensive identification of mouse testis-specific lncRNAs was previously lacking.

Purpose of the Study:

  • To comprehensively identify and characterize long noncoding RNAs (lncRNAs) that are specific to the mouse testis.
  • To investigate the expression patterns and genomic context of these testis-specific lncRNAs.
  • To establish a foundational catalog for future research into lncRNA function in spermatogenesis.

Main Methods:

  • Microarray analysis of transcripts from multiple mouse tissues, including the testis.
  • Bioinformatic analysis to identify and categorize tissue-specific lncRNAs, particularly long intergenic ncRNAs (lincRNAs).
  • Genomic proximity analysis and Gene Ontology (GO) analysis of protein-coding genes near lincRNA loci.
  • Expression analysis in male germ cell lines and in vitro validation of selected lncRNAs.

Main Results:

  • The testis exhibited the highest proportion of tissue-specific lncRNAs (11%) compared to other examined tissues.
  • A significant number of testis-specific lncRNAs were identified as long intergenic ncRNAs (lincRNAs).
  • Testis-specific lincRNAs were frequently located near protein-coding genes involved in transcriptional regulation, and many showed germ cell-specific expression.

Conclusions:

  • This study presents the first comprehensive catalog of mouse testis-specific lncRNAs.
  • The identified lncRNAs are potential key players in spermatogenesis and testicular function.
  • This catalog serves as a crucial resource for future functional studies of lncRNAs in male reproduction.