Related Experiment Video
Updated: Feb 7, 2026

Thermal Limits Determination for Zooplankton Using a Heat Block
Published on: November 18, 2022
Automated measurement of upper thermal limits in small aquatic animals
Tim Burton1, Bettina Zeis2, Sigurd Einum3
1Centre for Biodiversity Dynamics, Department of Biology, Norwegian University of Science and Technology, Realfagbygget, NO-7491 Trondheim, Norway tim.burton@ntnu.no.
Abstract:
We present a method for automating the measurement of upper thermal limits in small aquatic organisms. Upper thermal limits are frequently defined by the cessation of movement at high temperature, with measurement being performed by manual observation. Consequently, estimates of upper thermal limits may be subject to error and bias, both within and among observers. Our method utilises video-based tracking software to record the movement of individuals when exposed to high, lethal temperatures. We develop an algorithm in the R computing language that can objectively identify the loss of locomotory function from tracking data. Using independent experimental data, we validate our approach by demonstrating the expected response in upper thermal limits to acclimation temperature.
Related Concept Videos
Limiting Reactant
The Number e as a Limit
Thermal expansion and Thermal stress: Problem Solving
To solve the problem, first, identify the known and unknown quantities. The initial length (L) of the bridge is 1275 m, the coefficient of linear expansion (α) for steel is 12 x 10-6/°C, and the change in temperature (ΔT) is 55...
Types of Limits I
Limit Laws I
Arteries of the Upper Limbs

