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Updated: Feb 7, 2026

High-throughput Nitrobenzoxadiazole-labeled Cholesterol Efflux Assay
Published on: January 7, 2019
Endothelial-cell inflammation and damage by reactive oxygen species are prevented by propofol via ABCA1-mediated
Chih-Peng Hsu1, Chih-Hung Lin2, Chan-Yen Kuo3,4
1Department of Cardiology, Chang Bing Show Chwan Memorial Hospital, Changhua, Taiwan.
Abstract:
Background: Cholesterol efflux efficiency, reactive oxygen species, and inflammation are closely related to cardiovascular diseases. Our aim was to investigate the effect of propofol on cholesterol-loaded rat aortic endothelial cells after high-density lipoprotein treatment in vitro. Methods and Results: The results showed that propofol promoted cholesterol efflux and ameliorated inflammation and reactive oxygen species overproduction according to the analysis of p65 nuclear translocation and a 2',7'-dichlorofluorescin diacetate assay, respectively. Conclusions: These results provide a possible explanation for the anti-inflammatory, antioxidant, and cholesterol efflux-promoting effects of propofol on rat aortic endothelial cells after incubation with high-density lipoprotein.
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