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Metabolic engineering strategies for enhanced shikimate biosynthesis: current scenario and future developments
Muhammad Bilal1,2, Songwei Wang1, Hafiz M N Iqbal3
1State Key Laboratory of Microbial Metabolism, School of Life Sciences and Biotechnology, Shanghai Jiao Tong University, Shanghai, 200240, China.
Applied Microbiology and Biotechnology
|July 18, 2018
Summary
Microbial biosynthesis offers a sustainable alternative to plant-based shikimic acid production. Metabolic engineering and synthetic biology enhance production titers for this key pharmaceutical intermediate.
Area of Science:
- Biotechnology
- Metabolic Engineering
- Synthetic Biology
Background:
- Shikimic acid is a crucial precursor for pharmaceuticals like oseltamivir.
- Current plant-based extraction from Illicium verum is costly and unstable.
- Microbial biosynthesis presents a sustainable and cost-effective alternative.
Purpose of the Study:
- To review enhanced microbial biosynthesis of shikimic acid.
- To explore traditional and novel engineering strategies for increased production.
- To discuss shikimic acid derivatives and their production.
Main Methods:
- Metabolic engineering to optimize precursor levels and enzyme activity.
- Synthetic and systems biology for constructing high-yield strains.
- Bioprocess engineering strategies for efficient production.
Main Results:
- Metabolic engineering significantly elevates shikimic acid production titers.
- Novel technologies enable the construction of superior producing strains.
- Engineering strategies are applicable to diverse bacterial hosts.
Conclusions:
- Microbial biosynthesis is a viable and eco-friendly route for shikimic acid.
- Advanced engineering approaches promise further production enhancements.
- Shikimic acid derivatives can be efficiently produced using engineered microbes.
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