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[Interference of Lumican Regulates the Invasion and Migration of Liver Cancer Cells]

Qi-Ming Mu1, Wei He1, Gui-Min Hou1

  • 1Department of Hepatobiliary and Pancreatic Surgery,the 363 Hospital of Aviation Industry,Chengdu 610041,China.

Sichuan Da Xue Xue Bao. Yi Xue Ban = Journal of Sichuan University. Medical Science Edition
|July 18, 2018
PubMed
Abstract

Insights

Silencing lumican in liver cancer cells reduces their invasion and migration. This occurs by inhibiting the ERK1/JNK pathway, offering potential therapeutic targets for liver cancer.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cell Biology

Background:

  • Lumican is upregulated in liver cancer cells (HepG2, MHCC97H) compared to normal hepatocytes.
  • Increased lumican expression correlates with enhanced cancer cell invasion and migration.

Purpose of the Study:

  • To investigate the role of lumican in liver cancer progression.
  • To determine the effect of lumican silencing on cancer cell invasion and migration.

Main Methods:

  • Lumican was silenced using short hairpin RNA (shRNA) in HepG2 and MHCC97H cell lines.
  • Quantitative real-time PCR (qRT-PCR) and Western blotting were used to assess lumican mRNA and protein levels.
  • Cell invasion and migration were evaluated using Transwell and wound healing assays, respectively.

Main Results:

  • shRNA-mediated silencing of lumican significantly reduced its mRNA and protein expression in liver cancer cells.
  • Silencing lumican attenuated cancer cell invasion and migration.
  • Downregulation of lumican decreased the expression of matrix metalloproteinase-9 (MMP-9) and vascular endothelial growth factor (VEGF).
  • The activation of the ERK1/JNK signaling pathway, indicated by phosphorylated ERK1 (p-ERK1) and phosphorylated JNK (p-JNK), was reduced.

Conclusions:

  • Lumican silencing effectively inhibits liver cancer cell invasion and migration.
  • The inhibitory effect is mediated through the suppression of the ERK1/JNK signaling pathway.
  • Targeting lumican presents a potential therapeutic strategy for managing liver cancer.
Keywords:
ERK1/JNK pathwayInvasionLiver cancerMigrationlumicanshRNA

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