Related Experiment Video For Epithelial-to-mesenchymal transition
Updated: Feb 7, 2026

Induction and Analysis of Epithelial to Mesenchymal Transition
Published on: August 27, 2013
[IL-8 Induces Epithelial-to-mesenchymal Transition of Ovarian Carcinoma Cells: a Preliminary Study]
Shi-Chao Wang1,2, Hui-Hui Fu2, Ji-Rui Wen2
1School of Basic Medical Science,Xinjiang Medical University,Urumqi 830011, China.
Objective:
To explore the effect of IL-8 on the epithelial-to-mesenchymal transition (EMT) in ovarian cancer,which will provide experimental basis for revealing related molecular mechanism in malignant metastasis of ovarian cancer.
Methods:
The migration of ovarian cancer cell line SKOV3 cells was explored with Real time label free cell analysis (RTCA) after treatment with recombinant human IL-8.SKOV3 cells were co-cultured with IL-8 for 48 h,proteins involved in EMT were investigated via Western blot to explore the effect of IL-8 on the activation of the EMT. Invasion of SKOV3 cells after treatment with IL-8 were evaluated by transwell assay.
Results:
According to the results of RTCA,after treatment with IL-8 for 48 h,the migration of SKOV3 cells was in platform phase. The treatment of IL-8 unregulated vimentin and snail and downregulated E-cadherin,which suggested that IL-8 induced EMT in ovarian cancer. The results of transwell test showed that invasive ability of IL-8 pretreated SKOV3 cells was enhanced (P<0.05).
Conclusion:
IL-8 can induce the EMT of ovarian cancer and enhance the invasion and migration of ovarian cancer.
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Properties of Transition Metals
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