Related Experiment Video
Updated: Feb 7, 2026

Monitoring Dynamic Changes In Mitochondrial Calcium Levels During Apoptosis Using A Genetically Encoded Calcium Sensor
Published on: April 1, 2011
Long-term imaging of calcium dynamics using genetically encoded calcium indicators and automatic tracking of cultured
Igor Razlivanov1, Teresa Liew1, Eira Watts Moore1
1Department of Biomedical Sciences, The University of Sheffield, Western Bank, Sheffield, S10 2TN, UK.
Abstract:
Calcium dynamics is crucial for many signaling pathways and cell functions. Understanding how calcium regulates cell function often requires long-term imaging of calcium dynamics. Here we report a methodological approach of long-term (5-10 h) imaging of calcium dynamics in cultured cells. The approach links calcium imaging using genetically encoded calcium indicators and semi-automatic tracking of individual cells. It can be used in a large variety of situations, ranging from the role of calcium in biological processes to cell heterogeneity and screening of drugs modifying signaling pathways.
More Related Videos
08:01Author Spotlight: Investigating Viral Disruption of Intestinal Epithelial Signaling – Research Insights and Future Directions
Published on: January 19, 2024
12:19Imaging Intracellular Ca2+ Signals in Striatal Astrocytes from Adult Mice Using Genetically-encoded Calcium Indicators
Published on: November 19, 2014
Related Concept Videos
Imaging Studies for Cardiovascular System VI: Calcium -Scoring CT
Skeleton and Calcium Homeostasis
Indicators
Roles of Electrolytes: Calcium and Phosphate
The calcium concentration in blood plasma is primarily...
Long-term Depression
Feedback Regulation of Calcium Concentration
Various transmembrane receptors, such as G protein-coupled receptors (GPCRs), elicit a response to extracellular signals by increasing cytosolic calcium. Activated GPCRs...