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Updated: Feb 7, 2026
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Author Spotlight: Advancements in Molecular Biomarker Testing for Non-Squamous Non-Small Cell Lung Cancer
Published on: September 8, 2023
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Oncogenic Function of a KIF5B-MET Fusion Variant in Non-Small Cell Lung Cancer
Chien-Hung Gow1, Yi-Nan Liu2, Huei-Ying Li3
1Department of Internal Medicine, Far Eastern Memorial Hospital, New Taipei City, Taiwan; Department of Internal Medicine, National Taiwan University Hospital and College of Medicine, National Taiwan University, Taipei, Taiwan; Department of Healthcare Information and Management, Ming-Chuan University, Taiwan.
Neoplasia (New York, N.Y.)
|July 18, 2018
Summary
The KIF5B-MET fusion drives cancer growth in lung tumors by activating key signaling pathways. MET inhibitors show promise in treating non-small cell lung cancer patients with this specific gene rearrangement.
Area of Science:
- Oncology
- Molecular Biology
- Genetics
Background:
- Kinesin family member 5b (KIF5B)-MET proto-oncogene, receptor tyrosine kinase (MET) rearrangement is found in lung adenocarcinoma.
- The oncogenic role of KIF5B-MET fusion requires further investigation.
Purpose of the Study:
- To screen for KIF5B-MET fusions in lung cancer patients.
- To evaluate the oncogenic function of KIF5B-MET.
- To assess the efficacy of MET inhibitors against KIF5B-MET driven tumors.
Main Methods:
- Reverse transcription-polymerase chain reaction (RT-PCR) for fusion screening.
- Next-generation sequencing (NGS) for breakpoint determination.
- In vitro (cell assays) and in vivo (xenograft models) functional studies.
- Treatment with specific MET inhibitors.
Main Results:
- Identified KIF5B-MET fusion in lung adenocarcinoma and pulmonary sarcomatoid carcinoma patients.
- KIF5B-MET overexpression increased cell proliferation, colony formation, and tumor growth in vivo.
- Fusion activated MET, STAT3, AKT, and ERK1/2 signaling pathways.
- MET inhibitors reduced proliferation, downstream signaling, and tumor growth.
Conclusions:
- KIF5B-MET fusion possesses oncogenic functions in cancer cells.
- This finding supports targeted therapy for non-small cell lung cancer patients with KIF5B-MET rearrangement.
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