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Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality
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Author Spotlight: Exploring Cellular Zinc Regulation Through ZnT1 Functionality

Published on: June 2, 2023

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ZIP8 induces monocyte adhesion to the aortas ex-vivo by regulating zinc influx

Gong Cheng1, Feng-Jun Chang2, Peng-Hua You2

  • 1Department of Cardiology, The First Affiliated Hospital of Xi'an Jiaotong University, China.

International Immunopharmacology
|July 18, 2018
PubMed

Monocytes recruited and adhering to the inflamed arteries are crucial for atherosclerosis development. Here, we report the role of zinc (Zn2+) homeostasis in monocyte adhesion and recruitment. By comparing the expression levels of Zn2+ transporters between non-adhering and adhering monocytes, we found that the Zn2+ importer ZIP8 was specifically upregulated in monocytes adhering to the aortas ex-vivo. Although the overexpression of ZIP8 increased the absorption of Zn2+, Fe2+ and Cd2+ in monocytes, only Zn2+ supplementation was demonstrated capable of promoting the adhesion of monocytes to endothelial monolayers in vitro. In addition, we confirmed the role of ZIP8-dependent Zn2+ influx in promoting monocyte adhesion to the aortas ex-vivo. More importantly, the enforced expression of ZIP8 increased monocyte adhesion and recruitment to the nascent atherosclerotic lesions in ApoE-/- mice. Overall, our results suggest that the Zn2+ influx in monocytes regulated by ZIP8 is a novel factor determining their adhesion and recruitment to atherosclerotic lesions, and that targeting ZIP8 or Zn2+ homeostasis may represent a novel strategy to interfere these activities.

Keywords:
AdhesionAtherosclerosisMonocyteZIP8Zinc

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