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Updated: Feb 7, 2026
Aseptic Laboratory Techniques: Plating Methods
Published on: May 11, 2012
Enteroviruses Associated with Aseptic Meningitis in Poland, 2011-2014
Magdalena Wieczorek1, Agnieszka Figas1, Arleta Krzysztoszek1
1National Institute of Public Health - National Institute of Hygiene, Department of Virology, Warsaw, Poland.
Abstract:
A 4-year study (2011-2014) of patients with meningitis was performed. Out of the 686 cerebrospinal fluid samples, 465 (67.8%) were posi-tive for eneteroviruses using RT-PCR and out of 334 clinical samples, 216 (64.7%) were positive for enteroviruses using cell culture methods. The highest detection rate was observed in the summer and autumn. In total, 185 enteroviruses were identified by using neutralization test. Echovirus 6 and 30 were the most common (41.7% and 37.5% respectively). The highest frequency of neurological infections (32.7%) occurred in children aged 5-9 years, mostly males (63.9%).
Insights
Enteroviruses are a common cause of meningitis, particularly in children aged 5-9. This study found Echovirus 6 and 30 to be the most prevalent types, with higher detection rates in summer and autumn.
Area of Science:
- Virology
- Epidemiology
- Neurology
Background:
- Meningitis is a serious infection affecting the central nervous system.
- Enteroviruses are a significant cause of viral meningitis, especially in pediatric populations.
- Understanding enterovirus epidemiology is crucial for public health interventions.
Purpose of the Study:
- To investigate the prevalence and types of enteroviruses causing meningitis.
- To identify demographic and seasonal patterns of enterovirus infections.
- To compare detection rates between RT-PCR and cell culture methods.
Main Methods:
- Retrospective analysis of 686 cerebrospinal fluid (CSF) samples and 334 clinical samples from 2011-2014.
- Detection of enteroviruses using reverse transcription-polymerase chain reaction (RT-PCR) and cell culture.
- Identification of enterovirus types using neutralization tests.
Main Results:
- Enteroviruses were detected in 67.8% of CSF samples (RT-PCR) and 64.7% of clinical samples (cell culture).
- Echovirus 6 (41.7%) and Echovirus 30 (37.5%) were the most common identified types.
- The highest infection rates occurred in children aged 5-9 years (32.7%), predominantly males (63.9%), with peak incidence in summer and autumn.
Conclusions:
- Enteroviruses are a major causative agent of meningitis, with Echovirus 6 and 30 being predominant.
- RT-PCR and cell culture are effective diagnostic methods for enterovirus detection in meningitis.
- Targeted surveillance and prevention strategies during summer and autumn may be beneficial, especially for pediatric populations.
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