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Detection of Functional Matrix Metalloproteinases by Zymography
Published on: November 8, 2010
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Rational Engineering a Multichannel Upconversion Sensor for Multiplex Detection of Matrix Metalloproteinase
Sunan Cao1, Zhi Li1, Jinyuan Zhao1
1The Key Lab of Health Chemistry and Molecular Diagnosis of Suzhou, College of Chemistry, Chemical Engineering and Materials Science , Soochow University , Suzhou , 215123 , P. R. China.
ACS Sensors
|July 18, 2018
Summary
This study introduces a novel optical sensor using upconversion nanoparticles for simultaneously detecting two cancer-related proteases, matrix metalloproteinases (MMP-2 and MMP-7). This multiplex sensing approach minimizes signal crosstalk, improving cancer diagnostics and drug discovery.
Area of Science:
- Biomedical Engineering
- Nanotechnology
- Analytical Chemistry
Background:
- Optical sensing of cancer-relevant proteases is crucial for diagnostics, prognosis, and drug discovery.
- Multiplex sensing enhances predictive accuracy but faces challenges with fluorescence signal crosstalk.
- Matrix metalloproteinases (MMP-2 and MMP-7) are key targets in cancer research.
Purpose of the Study:
- To develop a multichannel optical sensor for multiplex ratiometric sensing of MMP-2 and MMP-7 proteolytic activities.
- To overcome the limitations of signal crosstalk in single-assay multiplex detection.
- To establish a versatile platform for detecting various proteases.
Main Methods:
- Fabrication of a NaYF4:Gd3+/Yb3+@NaYF4:Yb3+/Tm3+/Er3+ core-shell structure for multicolor emission.
- Utilizing luminescence resonance energy transfer (LRET) between nanoparticle dopants and surface fluorophores.
- Constructing the sensor using polyhistidine peptides conjugated with FITC and TAMRA for specific protease recognition.
Main Results:
- The sensor demonstrated specific blue and green emission activated by MMP-7 and MMP-2, respectively, upon peptide cleavage.
- Red emission served as an internal reference for ratiometric sensing, enhancing accuracy.
- The developed sensor exhibited high specificity and sensitivity with minimal signal crosstalk and cross-reactivity.
Conclusions:
- A novel multichannel optical sensor based on upconversion nanoparticles was successfully developed for multiplex ratiometric sensing of MMP-2 and MMP-7.
- The sensor effectively minimizes fluorescence signal crosstalk, offering improved accuracy for protease detection.
- This platform holds potential for broad applications in multiplex protease detection for cancer diagnostics and drug discovery.
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