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Updated: Feb 7, 2026
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Whole-Mount Staining, Visualization, and Analysis of Fungiform, Circumvallate, and Palate Taste Buds
Published on: February 11, 2021
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Radially patterned cell behaviours during tube budding from an epithelium
Yara E Sanchez-Corrales1, Guy B Blanchard2, Katja Röper1
1MRC Laboratory of Molecular Biology, Cambridge, United Kingdom.
Elife
|July 18, 2018
Summary
Tube budding in Drosophila embryos involves cell intercalation alongside apical constriction. This study reveals radially patterned cell behaviors and myosin enrichment driving early salivary gland tube formation.
Area of Science:
- Developmental Biology
- Cell Biology
- Morphogenesis
Background:
- Tubular organ formation from epithelial sheets is crucial for development.
- Understanding the cellular mechanisms of invagination is key to developmental biology.
Purpose of the Study:
- To investigate the cellular behaviors driving early salivary gland tube invagination in Drosophila.
- To elucidate the role of cell intercalation and apical constriction in tube budding.
Main Methods:
- Live-imaging of Drosophila embryos.
- Novel morphometric analysis in 2D and 3D.
- Analysis of junctional myosin distribution and cell neighbor exchanges.
Main Results:
- Radially oriented cell intercalation significantly contributes to early invagination.
- Apical constriction causes cell wedging near the invagination pit.
- Cells further from the pit exhibit circumferential interleaving, driven by apical behaviors and myosin enrichment.
Conclusions:
- Tube budding requires coordinated, radially patterned cell behaviors, including intercalation and apical constriction.
- Apical myosin distribution and cell behaviors are tightly linked to invagination mechanics.
- This study highlights the interplay between mechanical forces and cell behaviors in morphogenesis.
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