Two-Photon Microscopy with a Double-Wavelength Metasurface Objective Lens

Area of Science:

• Optics
• Biomedical Imaging
• Materials Science

Background:

• Two-photon microscopy is crucial for deep-tissue imaging in life sciences.
• Compact microscopes are needed for in vivo deep brain imaging.
• Dielectric metasurfaces offer miniaturized optical components like lenses.

Purpose of the Study:

• To demonstrate two-photon microscopy using a novel double-wavelength metasurface lens.
• To assess the performance of metasurface lenses for deep brain imaging applications.
• To explore the potential of metasurfaces for creating ultracompact microscopes.

Main Methods:

• Experimental demonstration of two-photon microscopy.
• Design and implementation of a double-wavelength metasurface lens focusing 820 nm and 605 nm light to the same focal distance.
• Comparison of image quality with conventional objective lenses.

Main Results:

• Achieved comparable image quality to conventional objective lenses in two-photon microscopy.
• Demonstrated the feasibility of using metasurface lenses for ultracompact microscope designs.
• Highlighted the potential for further development, including tunable metasurfaces for volumetric imaging.

Conclusions:

• Metasurface lenses can enable ultracompact two-photon microscopes with performance comparable to existing systems.
• This technology advances in vivo deep brain imaging capabilities.
• Tunable metasurface lenses offer future possibilities for enhanced volumetric imaging.