Related Experiment Video

Updated: Feb 7, 2026

Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase
07:50

Detection and Removal of Nuclease Contamination During Purification of Recombinant Prototype Foamy Virus Integrase

Published on: December 8, 2017

7.6K

Prototype foamy virus integrase is promiscuous for target choice

R M Mackler1, M A Lopez1, M J Osterhage1

  • 1Department of Cancer Biology and Genetics, Ohio State University College of Medicine, 460 West 12(th)Ave, Columbus, OH, 43210, USA.

Biochemical and Biophysical Research Communications
|July 19, 2018
PubMed

Keywords:
Divalent cationEnzymologyIntegrasePrototype foamy virusRetrovirus

More Related Videos

Assembly and Purification of Prototype Foamy Virus Intasomes10:20

Assembly and Purification of Prototype Foamy Virus Intasomes

Published on: March 19, 2018

6.0K
Choice and No-Choice Assays for Testing the Resistance of A. thaliana to Chewing Insects08:24

Choice and No-Choice Assays for Testing the Resistance of A. thaliana to Chewing Insects

Published on: May 14, 2008

17.6K

Related Concept Videos

Concepts and Prototypes01:24

Concepts and Prototypes

The human nervous system handles vast amounts of information by translating sensory stimuli into neural impulses, which the brain processes, creating thoughts expressed through language or stored as memories. The brain also synthesizes information from emotions and memories, which significantly influence thoughts and behaviors. This intricate process creates a comprehensive mental picture.
The brain organizes this information using concepts, which are mental categories grouping linguistic data,...
548
Mate Choice01:20

Mate Choice

Mate choice—the decision about whom to mate with—is a type of natural selection, since animals must reproduce to pass down their genes. Mate choice is also called intersexual selection because the behavior occurs between the sexes.
11.8K
What are Viruses?00:50

What are Viruses?

Overview
128.3K
Personal Choice and Fate Attributions01:19

Personal Choice and Fate Attributions

Some individuals interpret life events as a consequence of their personal choices and actions, while others believe that outcomes are dictated by fate or destiny. This divergence in perspective has been examined in psychological and cross-cultural studies, particularly in relation to religious faith and cultural beliefs about causality.Fate and Personal ResponsibilityPeople who emphasize personal responsibility view events as direct consequences of their decisions. For instance, breaking a leg...
178
Factors Affecting α-Alkylation of Ketones: Choice of Base01:10

Factors Affecting α-Alkylation of Ketones: Choice of Base

α-Alkylation of ketones is achieved in the presence of alkyl halides and a base. The reaction proceeds via the formation of an enolate ion followed by nucleophilic substitution. The choice of base employed is essential as it is the key factor in determining the reaction outcome.
The reaction involving bases like EtO− whose conjugate acid EtOH (pKa = 15.9) is stronger than the ketone (pKa = 19.2) results in an equilibrium mixture with higher ketone concentration. As a consequence,...
3.6K
Introduction to Virus01:28

Introduction to Virus

Viruses are unique biological entities that blur the boundary between living and non-living systems. Although they lack cellular structure and metabolic processes, they can exhibit characteristics of life when infecting a host. Their defining feature is a nucleic acid core, composed of either DNA or RNA, encapsulated within a protein coat called a capsid. This simple structure allows them to invade host cells and use their machinery for replication efficiently.Viral Structure and...
1.7K