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Related Concept Videos

CRISPR01:59

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
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Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
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When a force is applied on a body, it undergoes deformation. In order to restore the body to its original shape and/or size, an opposite or restoring force is generated within the body. This restoring force is equal to the magnitude of the applied force, but acts in the opposite direction. The amount of this restoring force developed per unit area of the body is called stress. Stress is a tensor quantity and has the SI unit pascal. Stress can be separated into four broad categories depending...
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The general state of stress within a material can be accurately depicted using a stress tensor. This tensor encapsulates the internal forces distributed within a material subjected to external forces or deformations.
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Purification of Ubiquitinated p53 Proteins from Mammalian Cells
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CRISPR: Stressed about p53?

Miguel Foronda1, Lukas E Dow2

  • 1Sandra and Edward Meyer Cancer Center, Department of Medicine, Division of Hematology and Medical Oncology, Weill Cornell Medicine, New York, NY 10021, USA.

Trends in Molecular Medicine
|July 19, 2018
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Summary
This summary is machine-generated.

CRISPR genome editing can trigger cell stress responses, impacting genetic manipulation outcomes. Understanding these p53-mediated effects is crucial for improving in vitro and in vivo gene editing efficiency.

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Area of Science:

  • Molecular Biology
  • Genetics
  • Cell Biology

Background:

  • CRISPR-Cas9 is a powerful genome editing tool.
  • Variability in CRISPR editing efficiency across cell types is observed.
  • The underlying mechanisms for this variability are not fully understood.

Purpose of the Study:

  • To investigate the cellular responses to CRISPR-mediated genome editing.
  • To explore the role of the p53 pathway in CRISPR editing outcomes.
  • To identify factors influencing genome editing effectiveness.

Main Methods:

  • Utilized CRISPR-Cas9 technology for genome editing experiments.
  • Analyzed cellular responses including stress markers and cell-cycle progression.
  • Compared editing outcomes across different cell types.

Main Results:

  • CRISPR editing can induce a p53-mediated stress response.
  • This stress response can lead to cell-cycle arrest.
  • Observed dissimilar outcomes in genetic manipulation across cell types.

Conclusions:

  • The p53 pathway activation is a key factor in CRISPR editing variability.
  • Understanding these cellular responses is essential for optimizing genome editing.
  • Further research is needed to enhance in vitro and in vivo CRISPR applications.