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Updated: Feb 7, 2026

Two-layered Membrane Sandwich Method for Laodelphax striatellus Saliva Collection
Published on: August 27, 2021
Cry78Aa, a novel Bacillus thuringiensis insecticidal protein with activity against Laodelphax striatellus and
Yinglong Wang1, Yonglei Liu2, Jie Zhang3
1School of Life Science, Northeast Agricultural University, Harbin 150030, PR China; State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, PR China.
Abstract:
Transgenic plants expressing insecticidal proteins originating from Bacillus thuringiensis (Bt) have successfully been used to control lepidopteran and coleopteran pests with chewing mouthparts. However, only a handful of Bt proteins have been identified that have bioactivity against sap sucking pests (Hemiptera), including aphids, whiteflies, plant bugs and planthoppers. A novel Bt insecticidal protein with significant toxicity against a hemipteran insect pest is described here. The gene encoding the 359 amino acid, 40.7 kDa protein was cloned from strain C9F1. After expression and purification of the toxin, its median lethal concentration (LC50) values against Laodelphax striatellus and Nilaparvata lugens were determined as 6.89 μg/mL and 15.78 μg/mL respectively. Analysis of the toxin sequence revealed the presence of both Toxin_10 and Ricin_B_Lectin domains.
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