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Related Concept Videos

Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

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Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
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Cells are the smallest and basic units of life, whether it is a single cell that forms the entire organism, e.g., in a bacterium or trillions of them, e.g., in humans. No matter what organism a cell is a part of, they share specific characteristics.
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Cancer arises from mutations in the critical genes that allow healthy cells to escape cell cycle regulation and acquire the ability to proliferate indefinitely. Though originating from a single mutation event in one of the originator cells, cancer progresses when the mutant cell lines continue to gain more and more mutations, and finally, become malignant. For example, chronic myelogenous leukemia (CML) develops initially as a non-lethal increase in white blood cells, which progressively...
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The cell is chemically composed of water, organic molecules and inorganic ions.
Water
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A battery is a galvanic cell that is used as a source of electrical power for specific applications. Modern batteries exist in a multitude of forms to accommodate various applications, from tiny button batteries such as those that power wristwatches to the very large batteries used to supply backup energy to municipal power grids. Some batteries are designed for single-use applications and cannot be recharged (primary cells), while others are based on conveniently reversible cell reactions that...
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Spontaneous Chemical Reactions
Spontaneous redox reactions occur abundantly in nature. The chemical reaction occurring in a disposable AA battery powering our remote controls is one such example of a spontaneous redox reaction. Another example is the immersion of coiled copper wire into an aqueous silver nitrate solution. The reaction shows a gradual, visually impressive color change from colorless to bright blue and the formation of a grey precipitate on the copper wire. In this experiment,...
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Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
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A Universal Live Cell Barcoding-Platform for Multiplexed Human Single Cell Analysis.

Felix J Hartmann1, Erin F Simonds2, Sean C Bendall3

  • 1Department of Pathology, School of Medicine, Stanford University, Palo Alto, CA, USA.

Scientific Reports
|July 19, 2018
PubMed
Summary
This summary is machine-generated.

This study introduces a new antibody-based platform for live-cell barcoding in mass cytometry (CyTOF). This method enhances assay efficiency and enables multiplexing of diverse human cells like immune, stem, and tumor cells.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Immunology

Background:

  • Single-cell barcoding improves assay efficiency by processing multiple samples simultaneously.
  • Current methods face challenges in barcoding live cells and accommodating diverse cell types for downstream analysis.

Purpose of the Study:

  • To develop a robust, antibody-based platform for live-cell barcoding in mass cytometry (CyTOF).
  • To enable flexible multiplexing of various human cell types within a single assay.
  • To enhance downstream assay performance and reduce technical variability.

Main Methods:

  • Developed a novel platform using platinum-conjugated antibodies targeting MHC class I (beta-2-microglobulin) and CD298.
  • Utilized a palladium-based covalent viability reagent compatible with the barcoding strategy.
  • Applied the platform to multiplex human immune cells, stem cells, and tumor cells.

Main Results:

  • Successfully demonstrated robust barcoding of live human cells for mass cytometry.
  • Achieved multiplexing of diverse cell types, including immune, stem, and tumor cells, in a single assay.
  • Validated a novel palladium-based viability reagent for compatibility with the barcoding approach.

Conclusions:

  • The developed platform enables efficient, live-cell barcoding for mass cytometry across multiple human sample types.
  • This strategy provides a versatile scheme for multiplexed human single-cell assays.
  • The approach enhances assay efficiency and reduces technical variability in sample preparation and analysis.