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Outer Membrane Lipoprotein Lip40 Modulates Adherence, Colonization, and Virulence of Actinobacillus pleuropneumoniae

Jinlin Liu1, Yurou Cao1, Lulu Gao1

  • 1Hubei Key Laboratory of Genetic Regulation and Integrative Biology, College of Life Sciences, Central China Normal University, Wuhan, China.

Frontiers in Microbiology
|July 19, 2018
PubMed

Insights

The outer membrane lipoprotein Lip40 is crucial for the virulence of Actinobacillus pleuropneumoniae. Deleting Lip40 significantly reduced bacterial colonization and disease severity in pigs, highlighting its role in pathogenesis.

Area of Science:

  • Bacteriology
  • Microbial Pathogenesis
  • Veterinary Medicine

Background:

  • Bacterial lipoproteins are essential membrane proteins with diverse roles.
  • Many lipoproteins function as virulence factors in pathogenic bacteria.
  • Actinobacillus pleuropneumoniae causes significant economic losses in the swine industry.

Purpose of the Study:

  • To investigate the role of the outer membrane lipoprotein Lip40 in the pathogenesis of Actinobacillus pleuropneumoniae.
  • To determine Lip40's contribution to bacterial adherence, colonization, and disease development.

Main Methods:

  • Construction of a Lip40-deficient mutant (Δlip40) and a complemented strain (CΔlip40) of A. pleuropneumoniae.
  • In vitro adherence assays using St. Jude porcine lung cells.
  • In vivo colonization studies in mouse lungs.
  • Infection assays in pigs to evaluate clinical signs and lung lesions.

Main Results:

  • The Δlip40 mutant showed significantly reduced adherence to porcine lung cells compared to the wild type.
  • Bacterial colonization of mouse lung tissues was markedly impaired in the Δlip40 mutant.
  • Pigs infected with the Δlip40 mutant exhibited fewer clinical signs and reduced lung lesions.

Conclusions:

  • Lipoprotein Lip40 is a significant virulence factor for Actinobacillus pleuropneumoniae.
  • Lip40 plays a critical role in bacterial adherence, colonization, and the development of porcine pleuropneumonia.
  • Targeting Lip40 could be a potential strategy for controlling A. pleuropneumoniae infections.
Keywords:
Actinobacillus pleuropneumoniaeLip40adherencecolonizationvirulence

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