An immune-competent rat split thickness skin graft model: useful tools to develop new therapies to improve skin graft...

Skin grafting is the routine standard of care to manage third degree burns and problematic skin defects. Several commercially available dermal substitutes and biologic skin equivalents are placed in the wound bed to facilitate the healing process of the skin grafts, as well as to provide mechanical support for the cells to grow and to delay the contracture. To study pathology and develop new therapies, an immune-competent rat model is required. We have created two different skin graft animal models to mimic the clinical skin grafting operation, the dorsum skin grafting (DG) and inguinal skin grafting (IG). To create a recipient site, a full-thickness, round excision wound was created on the dorsum between rats' scapular angles, covered with DG or IG. Graft contraction was quantified and tissue was harvested on predetermined time points for analysis. Histologic staining was performed to differentiate between DG and IG. Collagen deposition was assessed with Masson's trichrome staining. Mast cells were detected with Toluidine blue. Macrophages were stained with CD68 immune. Vascularity was assessed with functional vessels numbers. Cell proliferation was assessed with Ki67 immune. This model has all the advantages of murine models, such as an abundance of genetic variants and applicable tools, low cost, and practical housing techniques, all of which will promote the development of new therapies and testing new biologic skin equivalents and dermal substitutes.