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Related Concept Videos

Review and Preview01:10

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In statistics, several tools are used to interpret the data. Measures of central tendency represent the characteristics of the data, such as mean, median, and mode. Additionally, measures of variance like standard deviation and range are used to find the spread of data from the mean. Relative standing measures the distance between data locations. Commonly used measures of relative standings are percentile, z score, and quartiles.
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Data are individual items of information obtained from a population or sample. Data may be classified as qualitative (categorical), quantitative continuous, or quantitative discrete. Because it is not practical to measure the entire population in a study, researchers use samples to represent the population. A random sample is a representative group from the population chosen by using a method that gives each individual in the population an equal chance of being included in the sample. Random...
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As the human population continues to grow and use resources, we must be mindful of our planet’s natural limits. Sustainable development provides a pathway to maintain and improve human life now while also ensuring that future generations will have the resources that they need. The long-term success of sustainability efforts rests on understanding the interplay between human actions and ecological systems.
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Methanol Independent Expression by Pichia Pastoris Employing De-repression Technologies
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New Developments in Pichia pastoris Expression System, Review and Update.

Roghayyeh Baghban1,2,3, Safar Farajnia3,4, Younes Ghasemi5

  • 1Medical Biotechnology Department, Faculty of Advanced Medical Science, Tabriz University of Medical Sciences, Tabriz, Iran.

Current Pharmaceutical Biotechnology
|July 19, 2018
PubMed
Summary

Pichia pastoris is a versatile host for producing complex proteins. Recent advancements in glycoengineering and expression systems enhance its utility for recombinant protein production in industrial and clinical applications.

Keywords:
Crispr/Cas9Pichia pastorisexpression systemgenome engineeringglycoengineeringrecombinant protein.

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Area of Science:

  • Biotechnology
  • Molecular Biology
  • Microbial Expression Systems

Background:

  • Pichia pastoris is a well-established host for recombinant protein production.
  • Emerging interest in P. pastoris is driven by its inherent advantages and recent technological advancements.
  • Its capacity for secretory and soluble expression of heterologous glycoproteins makes it suitable for complex eukaryotic proteins.

Purpose of the Study:

  • To review key characteristics of the Pichia pastoris expression system.
  • To highlight recent developments in glycoengineering, expression vectors, and promoters.
  • To emphasize P. pastoris as a versatile host for complex protein production.

Main Methods:

  • Review of P. pastoris host strains, vectors, and fermentation technologies.
  • Focus on strain engineering using CRISPR/Cas9 for human-like glycosylation.
  • Development of protease-deficient strains for improved protein yield.

Main Results:

  • Significant advancements in P. pastoris host strains, vectors, and fermentation.
  • CRISPR/Cas9 technology enables production of human-like glycoproteins.
  • Protease-deficient strains improve recombinant protein stability and yield.

Conclusions:

  • Pichia pastoris has evolved into a highly versatile expression system.
  • Recent developments facilitate the production of complex proteins for therapeutic and industrial use.
  • This progress has led to several proteins entering clinical trials or industrial processes.