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Related Experiment Videos

Extrachromosomal circular DNA and aging cells.

C K Lumpkin, J R McGill, K T Riabowol

    Advances in Experimental Medicine and Biology
    |January 1, 1985
    PubMed
    Summary

    Inter-Alu DNA sequences are amplified in extrachromosomal DNA circles during cell culture. This amplification is observed in older individuals' lymphocytes but not in younger ones, suggesting an age-related change.

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    Area of Science:

    • Genetics
    • Molecular Biology
    • Human Aging Research

    Background:

    • The human genome contains repetitive DNA sequences, including Alu elements.
    • Extrachromosomal DNA elements can arise and replicate within cells.
    • Cellular aging is associated with various molecular alterations.

    Purpose of the Study:

    • To investigate the amplification of Inter-Alu DNA sequences in extrachromosomal DNA.
    • To determine if Inter-Alu DNA circle amplification is age-dependent.

    Main Methods:

    • Serial passage of diploid fibroblasts in cell culture.
    • Analysis of extrachromosomal DNA, including covalently closed DNA circles.
    • Detection of Inter-Alu DNA circles in lymphocytes from different age groups.

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    Main Results:

    • Inter-Alu DNA sequences progressively amplified in extrachromosomal DNA circles during fibroblast culture.
    • A specific size class of Inter-Alu circles was amplified in lymphocytes from elderly donors.
    • These amplified Inter-Alu circles were not detected in lymphocytes from young donors.

    Conclusions:

    • Inter-Alu DNA circle amplification occurs during serial cell passage.
    • The amplification of a specific Inter-Alu DNA circle size class is associated with aging.
    • This finding may indicate a novel molecular marker for cellular aging.