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Peripheral-blood gene expression profiling studies for coronary artery disease and its severity in Xinjiang
Meng Liu1, Shubin Jiang2, Yu Ma3
1Cancer Prevention and Research Institute, The affiliated Tumor Hospital of Xinjiang Medical University, Urumqi, 830011, Xinjiang, China.
Insights
Gene expression in peripheral blood cells can indicate coronary artery disease (CAD) severity. This study identified 12 toll-like receptor signaling pathway genes that may predict severe CAD in the Xinjiang population.
Area of Science:
- Genomics
- Molecular Biology
- Cardiovascular Disease Research
Background:
- Gene expression alterations in peripheral blood cells are linked to coronary artery disease (CAD).
- The relationship between gene expression and CAD severity in the Xinjiang population remains uncharacterized.
Purpose of the Study:
- To investigate gene expression profiles in peripheral blood cells.
- To identify genes associated with CAD severity in the Xinjiang population.
- To explore the diagnostic potential of these genes for severe CAD.
Main Methods:
- Global gene expression profiling of peripheral blood from controls and CAD patients.
- Screening differentially expressed genes using linear mixed effects analysis.
- Functional enrichment analysis (Gene Ontology, KEGG) and pathway analysis.
- Receiver operating characteristic (ROC) analysis to assess predictive value.
Main Results:
- Significant enrichment of toll-like receptor signaling pathway, immune responses, and inflammation.
- Identified 12 genes associated with the toll-like receptor signaling pathway.
- These 12 genes showed potential to predict severe CAD (AUC=0.67).
Conclusions:
- 12 toll-like receptor signaling pathway genes in peripheral blood reflect CAD presence and severity.
- These genes may serve as biomarkers for CAD severity in the Xinjiang population.
Background:
Alterations in gene expression in peripheral blood cells play a curtail role in the presence and extent of coronary artery disease (CAD), but its severity reflected by gene expression alterations in peripheral blood cells is still unknown in Xinjiang population in China.
Methods:
Global gene expression profiling in peripheral blood was used to explore differentially expressed genes in coronary artery stenosis patients. RNA was extracted from peripheral blood of 9 controls without coronary stenosis and 21 cases with angiographically CAD. The extent of CAD severity was categorized angiographically as no CAD, mild CAD (20 to 50% luminal diameter stenosis [LDS]), moderate CAD (50 to 75% LDS) and severe CAD (≥75% LDS). Differentially expressed genes related with CAD severity from peripheral blood cells were screened by linear mixed effects analysis using the lme4 package in R. Then the differentially expressed genes that gradually up-regulated or down-regulated were enriched by Gene Ontology (GO) functional annotation and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis.
Results:
The most significantly enrichments were toll-like receptor signaling pathway, immune responses, translational processes, cellular growth, inflammation and metabolic processes. Combined with NCBI-GeneRIF and PubMed analysis, we focused on the 12 genes associated with toll-like receptor signaling pathway in the extent of coronary artery stenosis patients. Receiver operating characteristic (ROC) analysis of 12 genes associated with toll-receptor signaling pathway in the 236 CAD patients from GEO database demonstrated that 12 genes expression could predict severe CAD with an area under the curve of 0.67, sensitivity of 77.65% and specificity of 51.52%.
Conclusion:
These results suggest that 12 genes associated with toll-like receptor signaling pathway in peripheral-blood cells reflect the presence and extent of CAD severity in Xinjiang population in China.
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