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Fragility Assessment of Bovine Cortical Bone Using Scratch Tests
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Osterix regulates corticalization for longitudinal bone growth via integrin β3 expression.

Young Jae Moon1,2, Chi-Young Yun2, Hwajung Choi2

  • 1Departments of Biochemistry, Chonbuk National University Medical School, Jeonju, Jeonbuk, 54896, Republic of Korea.

Experimental & Molecular Medicine
|July 20, 2018
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Osterix protein is crucial for bone growth by controlling osteoblast migration and matrix coalescence. This study reveals Osterix regulates integrin beta3 (Itgb3) expression, essential for proper corticalization and longitudinal bone development.

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Area of Science:

  • Bone Biology
  • Developmental Biology
  • Molecular Genetics

Background:

  • Corticalization, the process of trabecular bone merging into the metaphyseal cortex, is vital for long bone elongation.
  • The molecular pathways governing corticalization remain largely unelucidated.

Purpose of the Study:

  • To investigate the molecular mechanisms controlling corticalization during longitudinal bone growth.
  • To determine the role of Osterix in regulating osteoblast function and bone development.

Main Methods:

  • Analysis of osteoblast-specific Osterix-knockout mice (Col-OMT) and integrin beta3 (Itgb3)-null mice.
  • Assessment of corticalization, osteoblast distribution, and femoral length.
  • Investigation of Osterix binding to the Itgb3 promoter and its effect on gene transcription.

Main Results:

  • Col-OMT mice showed delayed corticalization, impaired osteoblast migration, and reduced femoral length.
  • Osterix directly binds to the Itgb3 promoter, enhancing Itgb3 gene transcription in osteoblasts.
  • Itgb3-null mice exhibited defects in cortical bone separation and reduced femoral length.

Conclusions:

  • Osterix is a key regulator of corticalization and longitudinal bone growth through its control of integrin beta3 expression in osteoblasts.
  • Targeting osteoblast function during corticalization may offer therapeutic strategies for conditions like short stature.