Cyanobacterial blooms in oil-contaminated subtidal sediments revealed by integrated approaches

Cyanobacteria are important primary producers on the surface of oceans and are susceptible to oil spills. However, their tolerance to oil and their roles in the bioremediation of crude oil remain elusive. We analysed the response of microbial communities to a simulated oil spill in estuarine sediment microcosms under a series of oil concentrations (0, 25, 125, and 250 g kg^-1 dry wt.). Cyanobacterial blooms only occurred on the sediment surface in the low oil (LO, 25 g kg^-1 dry wt.) group, and cyanobacteria grew from very small amounts to enriched levels according to an internal mechanism. The dominant phylotypes enriched in the oil-contaminated sediments on day 35 were Leptolyngbya, Oscillatoria, Arthrospira (Spirulina), Geitlerinema and Cyanothece, and the majority were capable of fixing nitrogen. Gammaproteobacterial blooms occurred during the early stage, and Oceanospirillales dominated the sediment surface. The annotation of unassembled metatranscriptomic data revealed an increase in nitrogen metabolism, particularly photosynthesis (antenna proteins) during the later stage, together with depletion of fatty acid metabolism. In summary, high concentrations of crude oil are toxic to cyanobacteria but can facilitate the emergence of cyanobacterial aggregation at low concentrations (crude oil concentration < 25 g kg^-1 dry wt.).