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Ultrastructures of silver nanoparticles biosynthesized using endophytic fungi

Lamabam Sophiya Devi1, S R Joshi1

  • 1Microbiology Laboratory, Department of Biotechnology & Bioinformatics, North-Eastern Hill University, Shillong 793 022, Meghalaya, India.

Journal of Microscopy and Ultrastructure
|July 20, 2018
PubMed

Insights

Endophytic fungi from Potentilla fulgens L. were used to biosynthesize silver nanoparticles. Aspergillus tamarii PFL2 produced the smallest spherical nanoparticles, confirmed as crystalline by electron microscopy and EDS analysis.

Area of Science:

  • * Mycology and Nanotechnology
  • * Biosynthesis of metallic nanoparticles using microbial agents

Background:

  • * Endophytic fungi, microorganisms residing within plant tissues, offer a sustainable source for nanoparticle biosynthesis.
  • * Potentilla fulgens L. is an ethno-medicinal plant with potential for harboring novel microbial strains.
  • * Silver nanoparticles (AgNPs) exhibit diverse applications in medicine and materials science.

Purpose of the Study:

  • * To investigate the biosynthesis of silver nanoparticles using three endophytic fungi isolated from Potentilla fulgens L.
  • * To characterize the structural morphology and elemental composition of the biosynthesized silver nanoparticles.
  • * To compare the efficacy of different fungal strains in producing silver nanoparticles of varying sizes.

Main Methods:

  • * Isolation and identification of endophytic fungi: Aspergillus tamarii PFL2, Aspergillus niger PFR6, and Penicillium ochrochloron PFR8.
  • * Biosynthesis of silver nanoparticles using fungal extracts.
  • * Characterization using Scanning Electron Microscopy (SEM), Transmission Electron Microscopy (TEM), Energy Dispersive X-ray Spectroscopy (EDS), and Selected Area Electron Diffraction (SAED).

Main Results:

  • * Spherical silver nanoparticles were successfully synthesized by all three fungal strains.
  • * Aspergillus tamarii PFL2 yielded the smallest average particle size (3.5 ±3 nm).
  • * Electron microscopy and EDS confirmed the formation of crystalline silver nanoparticles.

Conclusions:

  • * Endophytic fungi, particularly Aspergillus tamarii PFL2, are effective agents for the biosynthesis of crystalline silver nanoparticles.
  • * The size of biosynthesized silver nanoparticles can be controlled by the choice of fungal strain.
  • * This study highlights a green and efficient method for producing silver nanoparticles for potential applications.
Keywords:
CrystallineElectron microscopyEndophytic fungiSilver nanoparticles

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