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Fluorenyl-Loaded Quatsome Nanostructured Fluorescent Probes
Xinglei Liu1, Antonio Ardizzone2, Binglin Sui1
1Department of Chemistry and Environmental Science, College of Science and Liberal Arts, New Jersey Institute of Technology, 323 Martin Luther King, Jr., Blvd., Newark, New Jersey 07102, United States.
ACS Omega
|July 20, 2018
Summary
Researchers developed stable, water-soluble quatsomes (QS) to deliver hydrophobic fluorene probes. These nanovesicles show selective lysosomal localization in cells, enabling time-dependent tracking for biological applications.
Area of Science:
- Nanotechnology
- Biomedical Engineering
- Materials Science
Background:
- Hydrophobic materials face challenges in biological delivery, limiting their therapeutic and diagnostic potential.
- Quatsomes (QS), vesicles from cholesterol and quaternary amines, offer a stable, water-soluble nanocarrier solution.
- Fluorene-based probes possess advantageous properties but require effective delivery systems.
Purpose of the Study:
- To synthesize and characterize a novel quatsome (QS) nanovesicle for encapsulating hydrophobic fluorene probes.
- To investigate the photophysical properties and colloidal stability of these nanostructured probes.
- To evaluate the cellular uptake and localization of quatsome-fluorene nanoprobes in cancer and kidney cell lines.
Main Methods:
- Synthesis and characterization of quatsomes (QS) using cholesterol and amphiphilic quaternary amines.
- Photophysical analysis via UV-Vis absorption and fluorescence spectroscopy.
- Morphological and stability studies using cryogenic transmission electron microscopy (cryo-TEM).
- Cellular distribution assays in HCT 116 and COS-7 cell lines.
Main Results:
- Successfully created stable, water-soluble quatsomes (QS) capable of nanostructuring hydrophobic fluorene probes.
- Cryo-TEM revealed a "patchy" vascular morphology for the fluorenyl-quatsome nanovesicles.
- Demonstrated selective localization of C-QS and M-QS in lysosomes of HCT 116 and COS-7 cells with high colocalization.
- Confirmed utility for time-dependent lysosomal tracking.
Conclusions:
- Quatsomes (QS) provide an effective platform for delivering hydrophobic probes in biological systems.
- Fluorenyl-quatsome nanoprobes exhibit promising characteristics for cellular imaging and diagnostics.
- The selective lysosomal targeting enables advanced applications like time-dependent tracking.
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