Related Experiment Video
Updated: Feb 7, 2026
09:11
Synthesis of Near-Infrared Emitting Gold Nanoclusters for Biological Applications
Published on: March 22, 2020
8.4K
Single Platform for Gene and Protein Expression Analyses Using Luminescent Gold Nanoclusters
Sunil Kumar Sailapu1, Deepanjalee Dutta1, Amaresh Kumar Sahoo1
1Centre for Nanotechnology, Department of Biosciences and Bioengineering, and Department of Chemistry, Indian Institute of Technology Guwahati, Guwahati 781 039, Assam, India.
ACS Omega
|July 20, 2018
Summary
This study introduces a rapid diagnostic platform using gold nanoclusters for gene and protein expression analysis. The developed benchtop device enables simultaneous multi-gene/protein detection, aiding in disease diagnostics.
Area of Science:
- Biotechnology
- Nanotechnology
- Molecular Diagnostics
Background:
- Current gene and protein expression studies require rapid diagnostic tools.
- Gold nanoclusters offer luminescent properties for signal generation in biological assays.
Purpose of the Study:
- To develop a versatile, rapid diagnostic platform for simultaneous gene and protein expression analysis.
- To synthesize luminescent gold nanoclusters on DNA and protein templates using a benchtop device.
Main Methods:
- Synthesis of gold nanoclusters on DNA and protein templates.
- Development of a benchtop device for reverse transcriptase polymerase chain reaction and array-based analyses.
- Application of the device for gene profiling in HeLa cancer cells and protein analysis of glutathione-S-transferase (GST) and GST-hGMCSF.
Main Results:
- A common, rapid, and versatile method for gold nanocluster synthesis on DNA/protein templates was established.
- The platform successfully performed simultaneous multi-gene/protein analysis.
- Gene profiling related to apoptosis and protein expression of GST and GST-hGMCSF were evaluated.
Conclusions:
- The developed device and methods provide a user-friendly approach for disease diagnostics.
- The platform's potential for extending healthcare facilities to remote locations was highlighted.
- Luminescent gold nanoclusters serve as effective signal-generating agents for rapid diagnostics.
Related Concept Videos
01:42What is Gene Expression?
196.9K
Overview
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
Gene expression is the process in which DNA directs the synthesis of functional products, that is, proteins. Cells can regulate gene expression at various stages. It allows organisms to generate different cell types and enables cells to adapt to internal and external factors.
Genetic Information Flows from DNA to RNA to Protein
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is made up of nucleotides and proteins consist of amino...
196.9K
01:36What is Gene Expression?
11.5K
A gene is a stretch of DNA that serves as the blueprint for functional RNAs and proteins. Since DNA is comprised of nucleotides and proteins are comprised of amino acids, a mediator is required to convert the information encoded in DNA into proteins. This mediator is the messenger RNA (mRNA). mRNA copies the blueprint from DNA by a process called transcription. In eukaryotes, transcription occurs in the nucleus by complementary base-pairing with the DNA template. The mRNA is then...
11.5K
01:58Cell Specific Gene Expression
16.6K
Multicellular organisms contain a variety of structurally and functionally distinct cell types, but the DNA in all the cells originated from the same parent cells. The differences in the cells can be attributed to the differential gene expression. Liver cells, whose functions include detoxification of blood, production of bile to metabolize fats, and synthesis of proteins essential for metabolism, must express a specific set of genes to perform their functions. Gene expression also varies with...
16.6K
01:58Cell Specific Gene Expression
5.6K
5.6K
02:35Chromatin Position Affects Gene Expression
24.9K
Chromatin is the massive complex of DNA and proteins packaged inside the nucleus. The complexity of chromatin folding and how it is packaged inside the nucleus greatly influences access to genetic information. Generally, the nucleus' periphery is considered transcriptionally repressive, while the cell's interior is considered a transcriptionally active area.
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
Topologically Associated Domains (TADs)
The 3-dimensional positioning of chromatin in the nucleus influences the...
24.9K
02:51mRNA Stability and Gene Expression
6.7K
The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
Cis-acting Elements involved in mRNA stability
6.7K