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Updated: Feb 7, 2026

Bioassays for Monitoring Insecticide Resistance
Published on: December 30, 2010
An overview of functional genomic tools in deciphering insecticide resistance
Rafael A Homem1, Thomas G Emyr Davies1
1Department of Biointeractions and Crop Protection, Rothamsted Research, Harpenden AL5 2JQ, UK.
Abstract:
In this short review, we highlight three functional genomic technologies that have recently been contributing to the understanding of the molecular mechanisms underpinning insecticide resistance: the GAL4/UAS system, a molecular tool used to express genes of interest in a spatiotemporal controlled manner; the RNAi system, which is used to knock-down gene expression; and the most recently developed gene editing tool, CRISPR/Cas9, which can be used to knock-out and knock-in sequences of interest.
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