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Demonstration of P-selectin expression and potential function in human corneal epithelial cells
Peter J Gillies1, Neil A Richardson1, Jennifer Walshe2
1School of Biomedical Sciences, Faculty of Health and the Institute of Health & Biomedical Innovation, Queensland University of Technology, 2 George Street, Brisbane, Queensland, 4000, Australia; Queensland Eye Institute, 140 Melbourne Street, South Brisbane, Queensland, 4101, Australia.
P-selectin (CD62P) is expressed in human corneal epithelial cells, playing a role in cell movement. Inhibiting its binding to PSGL-1 (CD162) significantly slowed corneal cell sheet closure in culture.
Area of Science:
- Ophthalmology
- Cell Biology
- Immunology
Background:
- P-selectin (CD62P) is a cell adhesion molecule.
- Its presence and function in human corneal epithelial cells were previously uncharacterized.
Purpose of the Study:
- To investigate the expression and function of P-selectin in human corneal epithelial cells.
- To explore the role of P-selectin in corneal epithelial cell migration.
Main Methods:
- Immunocytochemistry, Western blotting, RT-PCR, and immunohistochemistry were used to detect P-selectin and its ligand PSGL-1 (CD162).
- A selective P-selectin-PSGL-1 inhibitor (KF38789) was used in a cell culture gap-closure assay with HCE-T cells and primary corneal epithelial cells.
Main Results:
- P-selectin was detected in HCE-T cells and primary corneal epithelial cells, localized at cell-cell boundaries and migrating edges.
- PSGL-1 was also detected in corneal epithelial cells and limbal fibroblasts.
- KF38789 significantly inhibited gap closure in HCE-T cell cultures but not in fibroblast cultures.
Conclusions:
- Human corneal epithelial cells express P-selectin.
- P-selectin likely contributes to the migration of corneal epithelial cell sheets.
- Targeting P-selectin-PSGL-1 interactions may influence corneal wound healing.
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