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Updated: Feb 7, 2026

Intradermal Inoculation of Mycobacterium avium in the Mouse Ear
Published on: July 3, 2025
Mycobacterium avium complex bacteria remain viable in sputum during storage and refrigeration
Lian J Pennings1, Sanne Zweijpfenning2, Mike M Ruth1
1Department of Medical Microbiology, Radboud University Medical Center, PO Box 9101, 6500, HB, Nijmegen, the Netherlands.
Abstract:
Diagnostic mycobacteriology often involves shipping of samples to centralized laboratories. Using two quantitative culture techniques, we show that 7 days storage of sputum samples at room temperature or 4 °C does not affect the number of viable Mycobacterium avium complex bacteria. Storage at room temperature increases the chance of contamination.
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