Enzymatic one-step ring contraction for quinolone biosynthesis
Shinji Kishimoto1, Kodai Hara1, Hiroshi Hashimoto1
1Department of Pharmaceutical Sciences, University of Shizuoka, Shizuoka, 422-8526, Japan.
Abstract:
The 6,6-quinolone scaffolds on which viridicatin-type fungal alkaloids are built are frequently found in metabolites that display useful biological activities. Here we report in vitro and computational analyses leading to the discovery of a hemocyanin-like protein AsqI from the Aspergillus nidulans aspoquinolone biosynthetic pathway that forms viridicatins via a conversion of the cyclopenin-type 6,7-bicyclic system into the viridicatin-type 6,6-bicyclic core through elimination of carbon dioxide and methylamine through methyl isocyanate.
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