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Related Experiment Video

Updated: Feb 7, 2026

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

A Bioluminescent and Fluorescent Orthotopic Syngeneic Murine Model of Androgen-dependent and Castration-resistant Prostate Cancer

Published on: March 6, 2018

Androgenic modulation of AR-Vs.

Ana Caroline Hillebrand¹, Lolita Schneider Pizzolato¹, Gisele Branchini²

¹Laboratory of Molecular Endocrine and Tumoral Biology, Department of Physiology, Institute of Basic Sciences of Health, Universidade Federal do Rio Grande do Sul, Porto Alegre, Rio Grande do Sul, 90050-170, Brazil.

|July 21, 2018

Summary

Androgen receptor variants (AR-Vs) expression dynamically changes with androgen levels in prostate cancer cells. Understanding these AR-V dynamics is crucial for developing new prostate cancer therapies.

Keywords:

Androgen receptor Androgen receptor isoforms Androgen receptor variants Androgenic modulation Prostate cancer Steroids

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Area of Science:

Oncology
Molecular Biology
Endocrinology

Background:

Androgen receptor variants (AR-Vs) play a significant role in prostate cancer progression.
AR-Vs are investigated as potential biomarkers for resistance to androgen deprivation therapy in metastatic prostate cancer.

Purpose of the Study:

To investigate the dynamic changes in AR-Vs expression in response to androgen modulation.
To understand the role of AR-Vs in prostate cancer under varying androgenic conditions.

Main Methods:

Utilized the C4-2B prostate cancer cell line.
Exposed cells to varying levels of dihydrotestosterone (DHT) and flutamide.
Assessed mRNA and protein expression of AR-Vs using qPCR and immunohistochemistry.

Main Results:

High DHT levels were found to downregulate both full-length androgen receptor (AR-FL) and AR-Vs.
AR-Vs exhibited dynamic expression changes in response to androgen treatment, despite lacking a ligand-binding domain.
Flutamide treatment, alone or with low DHT, stimulated prostatic cell growth.

Conclusions:

AR-Vs demonstrate differential responses to androgenic modulation.
These findings enhance the understanding of AR-Vs' contribution to prostate carcinogenesis.
The study highlights the complex behavior of AR-Vs in the context of androgen signaling in prostate cancer.